Attachment and response of human fibroblast and breast cancer cells to three dimensional silicon microstructures of different geometries

Mehdi Nikkhah, Jeannine S. Strobl, Masoud Agah

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The paper reports the development of three dimensional (3-D) silicon microstructures and the utilization of these microenvironments for discriminating between normal fibroblast (HS68) and breast cancer cells (MDA-MB-231). These devices consist of arrays of microchambers connected with channels and were fabricated using a single-mask, single-isotropic-etch process. The behavior and response of normal fibroblast and breast cancer cells, two key cell types in human breast tumor microenvironments, were explored in terms of adhesion and growth in these artificial 3-D microenvironments having curved sidewalls. Breast cancer cells formed stable adhesions with the curved sidewalls however fibroblasts stretched and elongated their cytoskeleton and actin filaments inside the microchambers. Statistical analysis revealed that fibroblast cells grew on both flat silicon surfaces and inside the microchambers regardless of microchamber depth. However, the localization of breast cancer cells in these same substrates was dependent on the microchamber depth. After 72 h in culture, the ratio of the number of breast cancer cells on flat surfaces compared to breast cancer cells inside the microchambers was significantly decreased within the deeper microchambers; for microchambers having depths 88 μm less than 5% of the breast cancer cells grew on the flat surfaces. This behavior was sustained for 120 h, the longest time point examined. The results suggest that certain 3-D silicon microstructures have potential application as a tool to detect breast cancer cells and also as a platform for separating normal fibroblasts from breast cancer cells for cancer diagnosis applications.

Original languageEnglish (US)
Pages (from-to)429-441
Number of pages13
JournalBiomedical Microdevices
Volume11
Issue number2
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Silicon
Fibroblasts
Cells
Breast Neoplasms
Microstructure
Geometry
Adhesion
Tumor Microenvironment
Masks
Cytoskeleton
Actin Cytoskeleton
Tumors
Statistical methods
Equipment and Supplies

Keywords

  • Breast cancer
  • Cellular behavior
  • Isotropic microchambers
  • MEMS
  • Single mask

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

Cite this

Attachment and response of human fibroblast and breast cancer cells to three dimensional silicon microstructures of different geometries. / Nikkhah, Mehdi; Strobl, Jeannine S.; Agah, Masoud.

In: Biomedical Microdevices, Vol. 11, No. 2, 2009, p. 429-441.

Research output: Contribution to journalArticle

@article{3f679b7fe6ee45a7ad212dd49b598bfd,
title = "Attachment and response of human fibroblast and breast cancer cells to three dimensional silicon microstructures of different geometries",
abstract = "The paper reports the development of three dimensional (3-D) silicon microstructures and the utilization of these microenvironments for discriminating between normal fibroblast (HS68) and breast cancer cells (MDA-MB-231). These devices consist of arrays of microchambers connected with channels and were fabricated using a single-mask, single-isotropic-etch process. The behavior and response of normal fibroblast and breast cancer cells, two key cell types in human breast tumor microenvironments, were explored in terms of adhesion and growth in these artificial 3-D microenvironments having curved sidewalls. Breast cancer cells formed stable adhesions with the curved sidewalls however fibroblasts stretched and elongated their cytoskeleton and actin filaments inside the microchambers. Statistical analysis revealed that fibroblast cells grew on both flat silicon surfaces and inside the microchambers regardless of microchamber depth. However, the localization of breast cancer cells in these same substrates was dependent on the microchamber depth. After 72 h in culture, the ratio of the number of breast cancer cells on flat surfaces compared to breast cancer cells inside the microchambers was significantly decreased within the deeper microchambers; for microchambers having depths 88 μm less than 5{\%} of the breast cancer cells grew on the flat surfaces. This behavior was sustained for 120 h, the longest time point examined. The results suggest that certain 3-D silicon microstructures have potential application as a tool to detect breast cancer cells and also as a platform for separating normal fibroblasts from breast cancer cells for cancer diagnosis applications.",
keywords = "Breast cancer, Cellular behavior, Isotropic microchambers, MEMS, Single mask",
author = "Mehdi Nikkhah and Strobl, {Jeannine S.} and Masoud Agah",
year = "2009",
doi = "10.1007/s10544-008-9249-5",
language = "English (US)",
volume = "11",
pages = "429--441",
journal = "Biomedical Microdevices",
issn = "1387-2176",
publisher = "Kluwer Academic Publishers",
number = "2",

}

TY - JOUR

T1 - Attachment and response of human fibroblast and breast cancer cells to three dimensional silicon microstructures of different geometries

AU - Nikkhah, Mehdi

AU - Strobl, Jeannine S.

AU - Agah, Masoud

PY - 2009

Y1 - 2009

N2 - The paper reports the development of three dimensional (3-D) silicon microstructures and the utilization of these microenvironments for discriminating between normal fibroblast (HS68) and breast cancer cells (MDA-MB-231). These devices consist of arrays of microchambers connected with channels and were fabricated using a single-mask, single-isotropic-etch process. The behavior and response of normal fibroblast and breast cancer cells, two key cell types in human breast tumor microenvironments, were explored in terms of adhesion and growth in these artificial 3-D microenvironments having curved sidewalls. Breast cancer cells formed stable adhesions with the curved sidewalls however fibroblasts stretched and elongated their cytoskeleton and actin filaments inside the microchambers. Statistical analysis revealed that fibroblast cells grew on both flat silicon surfaces and inside the microchambers regardless of microchamber depth. However, the localization of breast cancer cells in these same substrates was dependent on the microchamber depth. After 72 h in culture, the ratio of the number of breast cancer cells on flat surfaces compared to breast cancer cells inside the microchambers was significantly decreased within the deeper microchambers; for microchambers having depths 88 μm less than 5% of the breast cancer cells grew on the flat surfaces. This behavior was sustained for 120 h, the longest time point examined. The results suggest that certain 3-D silicon microstructures have potential application as a tool to detect breast cancer cells and also as a platform for separating normal fibroblasts from breast cancer cells for cancer diagnosis applications.

AB - The paper reports the development of three dimensional (3-D) silicon microstructures and the utilization of these microenvironments for discriminating between normal fibroblast (HS68) and breast cancer cells (MDA-MB-231). These devices consist of arrays of microchambers connected with channels and were fabricated using a single-mask, single-isotropic-etch process. The behavior and response of normal fibroblast and breast cancer cells, two key cell types in human breast tumor microenvironments, were explored in terms of adhesion and growth in these artificial 3-D microenvironments having curved sidewalls. Breast cancer cells formed stable adhesions with the curved sidewalls however fibroblasts stretched and elongated their cytoskeleton and actin filaments inside the microchambers. Statistical analysis revealed that fibroblast cells grew on both flat silicon surfaces and inside the microchambers regardless of microchamber depth. However, the localization of breast cancer cells in these same substrates was dependent on the microchamber depth. After 72 h in culture, the ratio of the number of breast cancer cells on flat surfaces compared to breast cancer cells inside the microchambers was significantly decreased within the deeper microchambers; for microchambers having depths 88 μm less than 5% of the breast cancer cells grew on the flat surfaces. This behavior was sustained for 120 h, the longest time point examined. The results suggest that certain 3-D silicon microstructures have potential application as a tool to detect breast cancer cells and also as a platform for separating normal fibroblasts from breast cancer cells for cancer diagnosis applications.

KW - Breast cancer

KW - Cellular behavior

KW - Isotropic microchambers

KW - MEMS

KW - Single mask

UR - http://www.scopus.com/inward/record.url?scp=62649137026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62649137026&partnerID=8YFLogxK

U2 - 10.1007/s10544-008-9249-5

DO - 10.1007/s10544-008-9249-5

M3 - Article

C2 - 19058013

AN - SCOPUS:62649137026

VL - 11

SP - 429

EP - 441

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 1387-2176

IS - 2

ER -