Atomic force microscopy reveals kinks in the p53 response element DNA

P. Balagurumoorthy, Stuart Lindsay, Rodney E. Harrington

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

p53 is a 53 kDa nuclear phosphoprotein. Its function as a tumor suppressor critically lies in its ability to recognize its target DNA response elements as a tetramer. Here, we report the structural theme intrinsic to the response element DNA that governs this recognition phenomenon. The intrinsic flexibility or dynamic bending between two distinctly different, but naturally occurring p53 response elements has been compared by ring closure. Results show that DNA binding sites containing helically phased d(CATG.CATG) tetra-nucleotide sequences at the centers of quasi-dyad symmetry in each half-response site are more intrinsically flexible (i.e. preferentially bent under axial stress) than their d(CTTG.CTTG) counterparts. Intriguingly, p53 binding sites containing these more flexible d(CATG.CATG) sequence elements also exhibit a stronger tendency for tetrameric binding of the p53 DNA binding domain peptide. Examination of the shapes of DNA microcircles obtained by circularization of oligomers constructed from such flexible p53 target DNA sequences in tandem using MacMode atomic force microscopy directly revealed sequence-specific kinks in solution. The tetra-nucleotide sequence d(CATG.CATG) is highly conserved in most functional p53 response elements. Consequently, we propose that the sequence-specific kinks originating from d(CATG.CATG) sequences could be a common structural theme in p53 response elements and as evident from the results reported here, could be a determinant of binding site recognition by the p53 protein and the subsequent stability of the p53-DNA complex.

Original languageEnglish (US)
Pages (from-to)611-623
Number of pages13
JournalBiophysical Chemistry
Volume101-102
DOIs
StatePublished - Dec 10 2002

Keywords

  • Contour length
  • DNA flexibility
  • Kink
  • MacMode atomic force microscopy
  • p21 response element
  • p53

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Atomic force microscopy reveals kinks in the p53 response element DNA'. Together they form a unique fingerprint.

  • Cite this