Atomic force microscopy of reovirus dsRNA: A routine technique for length measurements

Yuri L. Lyubchenko, Bertram Jacobs, Stuart Lindsay

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA molecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 μm. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 μm. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.

Original languageEnglish (US)
Pages (from-to)3983-3986
Number of pages4
JournalNucleic Acids Research
Volume20
Issue number15
StatePublished - Aug 11 1992

Fingerprint

Double-Stranded RNA
Atomic Force Microscopy
RNA
Atomic force microscopy
Electron Microscopy
Molecules
Electron microscopy
Electrophoresis
Buffers
Gels
Mica
Statistical Analysis
Buffer
Display
Statistical methods
Display devices
mica

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Atomic force microscopy of reovirus dsRNA : A routine technique for length measurements. / Lyubchenko, Yuri L.; Jacobs, Bertram; Lindsay, Stuart.

In: Nucleic Acids Research, Vol. 20, No. 15, 11.08.1992, p. 3983-3986.

Research output: Contribution to journalArticle

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