Atomic force microscopy of long DNA: Imaging in air and under water

Yuri Lyubchenko, Lyuda Shlyakhtenko, Rodney Harrington, Patrick Oden, Stuart Lindsay

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

We have obtained striking atomic force microscopy images of the intact λ bacteriophage genome and of several λ restriction fragments both in air and under water. The DNA is unstained and the images are stable under continuous scanning for up to 30 min. Measured contour lengths of fully imaged restriction fragments and intact λ DNA are accurate to within a few percent. The key to this development is the use of a process for binding unmodified double-stranded DNA to chemically treated mica surfaces. This procedure leads to strong DNA attachment and yields high-quality images that are stable under repeated scanning, even with the sample submerged in water. This allows normal hydration conditions to be maintained during scanning and in addition leads to a general improvement of image quality. Both the lateral resolution and the contrast increase by a factor of ≈3 under water.

Original languageEnglish (US)
Pages (from-to)2137-2140
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number6
StatePublished - Mar 15 1993

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Atomic Force Microscopy
Air
Water
DNA
Quality Improvement
Bacteriophages
Genome

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Atomic force microscopy of long DNA : Imaging in air and under water. / Lyubchenko, Yuri; Shlyakhtenko, Lyuda; Harrington, Rodney; Oden, Patrick; Lindsay, Stuart.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 6, 15.03.1993, p. 2137-2140.

Research output: Contribution to journalArticle

Lyubchenko, Yuri ; Shlyakhtenko, Lyuda ; Harrington, Rodney ; Oden, Patrick ; Lindsay, Stuart. / Atomic force microscopy of long DNA : Imaging in air and under water. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 6. pp. 2137-2140.
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