Atomic force microscopy of DNA and bacteriophage in air, water and propanol: The role of adhesion forces

Y. L. Lyubchenko, P. I. Oden, D. Lampner, Stuart Lindsay, K. A. Dunker

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

We have developed a chemical treatment for the mica surface which allows biopolymers to be held in place for atomic force microscopy, even under water, using conventional, untreated force sensing tips. We Illustrate the procedure with images of λ DNA and fd phage. The phage adheres well enough to permit in situ Imaging of the adsorption process in water. These experiments yield a mean length for the phage of 883±72nm. This compares with a measured length of 883±33nm when the phage are imaged after drying following adsorption from water, showing that the effect of dehydration is quite small. Adhesion forces between the force sensing tip and the substrate and the sensing tip and the biomolecules are very different in the three media (air, water and propanol). The apparent height of the phage and the width and height of the DMA depends upon these adhesion forces quite strongly. In contrast, changing the Hookean spring force exerted by the scanning tip makes little difference. These results suggest that the chemical factors involved in adhesion can dominate atomic force images and that the composition of the scanning tip is at least as important a factor as its geometry.

Original languageEnglish (US)
Pages (from-to)1117-1123
Number of pages7
JournalNucleic Acids Research
Volume21
Issue number5
StatePublished - 1993

Fingerprint

1-Propanol
Bacteriophages
Atomic Force Microscopy
Propanol
Adhesion
Atomic force microscopy
DNA
Air
Water
Adsorption
Sensing
Bacteriophage M13
Biopolymers
Scanning
Dehydration
Mica
Biomolecules
Dynamic mechanical analysis
Drying
Imaging techniques

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Atomic force microscopy of DNA and bacteriophage in air, water and propanol : The role of adhesion forces. / Lyubchenko, Y. L.; Oden, P. I.; Lampner, D.; Lindsay, Stuart; Dunker, K. A.

In: Nucleic Acids Research, Vol. 21, No. 5, 1993, p. 1117-1123.

Research output: Contribution to journalArticle

Lyubchenko, YL, Oden, PI, Lampner, D, Lindsay, S & Dunker, KA 1993, 'Atomic force microscopy of DNA and bacteriophage in air, water and propanol: The role of adhesion forces', Nucleic Acids Research, vol. 21, no. 5, pp. 1117-1123.
Lyubchenko, Y. L. ; Oden, P. I. ; Lampner, D. ; Lindsay, Stuart ; Dunker, K. A. / Atomic force microscopy of DNA and bacteriophage in air, water and propanol : The role of adhesion forces. In: Nucleic Acids Research. 1993 ; Vol. 21, No. 5. pp. 1117-1123.
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