Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs

Ramanathan Kasimanickam, Kevin D. Pelzer, Vanmathy Kasimanickam, William S. Swecker, Craig Thatcher

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The objective was to determine relationships among classical semen characteristics, sperm chromatin structure assay (SCSA), lipid peroxidation and antioxidant enzymatic activity in ram-lamb semen. Fifty-seven ram-lambs were electroejaculated, and routine semen evaluation was conducted (as part of a breeding soundness evaluation). The percentage of sperm DNA fragmentation index (%DFI) and the percentage of sperm with abnormally high DNA stainability (HDS; immature spermatozoa) were determined by SCSA using the metachromatic properties of acridine orange. Semen was centrifuged at 800 x g for 15 min to separate spermatozoa and seminal plasma and the aliquots were stored at -70°C until analyzed. Lipid peroxidation, superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in seminal plasma and spermatozoa were measured by spectophotometric assays. The classical semen parameters were negatively related to lipid peroxidation and GPx activity in spermatozoa; motility and morphology were negatively related to %DFI (P < 0.05). Based on Kruskal-Wallis pair-wise comparison of median values among breeding soundness outcome groups, %DFI was lower in the satisfactory group compared to other groups (P < 0.05) and the lipid peroxidation and GPx activity in seminal plasma and spermatozoa were lower in satisfactory and questionable groups (P < 0.05). However, the SOD was lower in the unsatisfactory group (P < 0.05). In summary, classical semen parameters were negatively related to % DFI, lipid peroxidation and GPx activity in ram-lamb spermatozoa and seminal plasma. There were indications that SOD and GPx have crucial protective roles against the toxic effect of reactive oxygen species (ROS) in ram-lamb semen.

Original languageEnglish (US)
Pages (from-to)1407-1421
Number of pages15
JournalTheriogenology
Volume65
Issue number7
DOIs
StatePublished - Apr 15 2006
Externally publishedYes

Fingerprint

DNA fragmentation
DNA Fragmentation
Semen
rams
Lipid Peroxidation
Spermatozoa
semen
lipid peroxidation
lambs
Antioxidants
spermatozoa
antioxidants
glutathione peroxidase
Glutathione Peroxidase
seminal plasma
breeding soundness
superoxide dismutase
Superoxide Dismutase
chromatin
Chromatin

Keywords

  • Antioxidant enzyme
  • DFI
  • Lipid peroxidation
  • Spermatozoa

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs. / Kasimanickam, Ramanathan; Pelzer, Kevin D.; Kasimanickam, Vanmathy; Swecker, William S.; Thatcher, Craig.

In: Theriogenology, Vol. 65, No. 7, 15.04.2006, p. 1407-1421.

Research output: Contribution to journalArticle

Kasimanickam, Ramanathan ; Pelzer, Kevin D. ; Kasimanickam, Vanmathy ; Swecker, William S. ; Thatcher, Craig. / Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs. In: Theriogenology. 2006 ; Vol. 65, No. 7. pp. 1407-1421.
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