Assessment of the cytotoxicity of photocrosslinked dextran and hyaluronan-based hydrogels to vascular smooth muscle cells

J. Trudel, Stephen Massia

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

The cytotoxicity of polysaccharide-based hydrogels and solutions was studied in vitro after 48 h of indirect exposure of the materials with vascular smooth muscle cells. Dextran and/or hyaluronan were derivatized using glycidyl methacrylate, and hydrogels were formed in the presence of photoinitiators and ultraviolet radiation in multiwell inserts to avoid direct contact with cell monolayers. Observation of cell morphology indicated that dextran hydrogels, a blend of non-derivatized hyaluronan into dextran hydrogel, and a hyaluronan solution were highly cytocompatible. However, hydrogels made of derivatized hyaluronan were cytotoxic when compared to unexposed sham controls that contained multiwell inserts but no hydrogels. Results from quantitative assays for proliferation and viability corroborated the qualitative observations, and scrape wound assays revealed a significant increase in smooth muscles cell migration/proliferation after indirect exposure to several of the polysaccharide-based materials. Results from this study demonstrate that hydrogels made of dextran and hyaluronan solution show good cytocompatibility in vitro, making these degradable matrices interesting candidates for drug delivery purposes.

Original languageEnglish (US)
Pages (from-to)3299-3307
Number of pages9
JournalBiomaterials
Volume23
Issue number16
DOIs
StatePublished - Jun 22 2002

Keywords

  • Cytotoxicity
  • Dextran
  • Hyaluronan
  • Hydrogels
  • In vitro
  • Photoinitiators
  • Smooth muscle cells

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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