Assessing the reliability of 5S rRNA sequence data for phylogenetic analysis in green plants

Kelly Steele, Kent E. Holsinger, Robert K. Jansen, David W. Taylor

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Elimination of compensating substitutions in portions of 5S rRNA sequences that exhibit secondary structure and scoring of common gaps as homologous characters affects both the topology of phylogenetic estimates within green plants and measures of their reliability. Allowing for a transition/transversion bias, on the other hand, has little affect. Detailed analysis of these data results in phylogenetic estimates largely congruent with widely accepted hypotheses of relationships among green plants, but bootstrapping provides little statistical support for most of these groupings. The lack of statistical support is a direct result of both the small number of phylogenetically informative sites in the 5S rRNA molecule and the high rate of change at those positions that are free to vary. When considered in conjunction with earlier distance-based analyses of phylogeny that use 5S rRNA, these results demonstrate that phylogenetic estimates based only on analyses of 5S rRNA sequences must be viewed with considerable caution.

Original languageEnglish (US)
Pages (from-to)240-248
Number of pages9
JournalMolecular Biology and Evolution
Volume8
Issue number2
StatePublished - Mar 1991
Externally publishedYes

Fingerprint

Viridiplantae
Substitution reactions
Topology
ribosomal RNA
phylogenetics
Molecules
phylogeny
Phylogeny
bootstrapping
secondary structure
topology
substitution
data analysis
analysis

Keywords

  • 5S ribosomal RNA
  • Bootstrap analyses
  • Green plants
  • Phylogenetic analyses

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Assessing the reliability of 5S rRNA sequence data for phylogenetic analysis in green plants. / Steele, Kelly; Holsinger, Kent E.; Jansen, Robert K.; Taylor, David W.

In: Molecular Biology and Evolution, Vol. 8, No. 2, 03.1991, p. 240-248.

Research output: Contribution to journalArticle

Steele, Kelly ; Holsinger, Kent E. ; Jansen, Robert K. ; Taylor, David W. / Assessing the reliability of 5S rRNA sequence data for phylogenetic analysis in green plants. In: Molecular Biology and Evolution. 1991 ; Vol. 8, No. 2. pp. 240-248.
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