Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2

Umesh P. Yadav, Mearaj A. Shaikh, John Evers, Kamesh C. Regmi, Roberto Gaxiola, Brian G. Ayre

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Phloem loading and long-distance transport of photoassimilate from source leaves to sink organs are essential physiological processes that contribute to plant growth and yield. At a minimum, three steps are involved: phloem loading in source organs, transport along the phloem path, and phloem unloading in sink organs. Each of these can have variable rates contingent on the physiological state of the plant, and thereby influence the overall transport rate. In addition to these phloem transport steps, rates of photosynthesis and photosynthate movement in the pre-phloem path, as well as photosynthate utilization in post phloem tissues of sink organs also contribute to phloem transport. The protocol described here estimates carbon allocation along the entire path from initial carbon fixation to delivery to sink organs after a labeling pulse: [14C]CO2 is photoassimilated in source leaves and loading and transport of the 14C label to heterotrophic sink organs (roots) is quantified by scintillation counting. This method is flexible and can be adapted to quantify long-distance transport in many plant species.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages223-233
Number of pages11
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2014
ISSN (Print)1064-3745

Fingerprint

Phloem
Carbon
Physiological Phenomena
Carbon Cycle
Scintillation Counting
Photosynthesis

Keywords

  • C labeling
  • Carbon allocation
  • Phloem transport
  • Photoassimilate partitioning
  • Photosynthetic labeling
  • Source-sink relations
  • Sugar transport

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yadav, U. P., Shaikh, M. A., Evers, J., Regmi, K. C., Gaxiola, R., & Ayre, B. G. (2019). Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2 In Methods in Molecular Biology (pp. 223-233). (Methods in Molecular Biology; Vol. 2014). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9562-2_19

Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2 . / Yadav, Umesh P.; Shaikh, Mearaj A.; Evers, John; Regmi, Kamesh C.; Gaxiola, Roberto; Ayre, Brian G.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 223-233 (Methods in Molecular Biology; Vol. 2014).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yadav, UP, Shaikh, MA, Evers, J, Regmi, KC, Gaxiola, R & Ayre, BG 2019, Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2 in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2014, Humana Press Inc., pp. 223-233. https://doi.org/10.1007/978-1-4939-9562-2_19
Yadav UP, Shaikh MA, Evers J, Regmi KC, Gaxiola R, Ayre BG. Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2 In Methods in Molecular Biology. Humana Press Inc. 2019. p. 223-233. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9562-2_19
Yadav, Umesh P. ; Shaikh, Mearaj A. ; Evers, John ; Regmi, Kamesh C. ; Gaxiola, Roberto ; Ayre, Brian G. / Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2 Methods in Molecular Biology. Humana Press Inc., 2019. pp. 223-233 (Methods in Molecular Biology).
@inbook{79d7566b9e1a4e628cc5a6bcdb1258ff,
title = "Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2",
abstract = "Phloem loading and long-distance transport of photoassimilate from source leaves to sink organs are essential physiological processes that contribute to plant growth and yield. At a minimum, three steps are involved: phloem loading in source organs, transport along the phloem path, and phloem unloading in sink organs. Each of these can have variable rates contingent on the physiological state of the plant, and thereby influence the overall transport rate. In addition to these phloem transport steps, rates of photosynthesis and photosynthate movement in the pre-phloem path, as well as photosynthate utilization in post phloem tissues of sink organs also contribute to phloem transport. The protocol described here estimates carbon allocation along the entire path from initial carbon fixation to delivery to sink organs after a labeling pulse: [14C]CO2 is photoassimilated in source leaves and loading and transport of the 14C label to heterotrophic sink organs (roots) is quantified by scintillation counting. This method is flexible and can be adapted to quantify long-distance transport in many plant species.",
keywords = "C labeling, Carbon allocation, Phloem transport, Photoassimilate partitioning, Photosynthetic labeling, Source-sink relations, Sugar transport",
author = "Yadav, {Umesh P.} and Shaikh, {Mearaj A.} and John Evers and Regmi, {Kamesh C.} and Roberto Gaxiola and Ayre, {Brian G.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9562-2_19",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "223--233",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2

AU - Yadav, Umesh P.

AU - Shaikh, Mearaj A.

AU - Evers, John

AU - Regmi, Kamesh C.

AU - Gaxiola, Roberto

AU - Ayre, Brian G.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Phloem loading and long-distance transport of photoassimilate from source leaves to sink organs are essential physiological processes that contribute to plant growth and yield. At a minimum, three steps are involved: phloem loading in source organs, transport along the phloem path, and phloem unloading in sink organs. Each of these can have variable rates contingent on the physiological state of the plant, and thereby influence the overall transport rate. In addition to these phloem transport steps, rates of photosynthesis and photosynthate movement in the pre-phloem path, as well as photosynthate utilization in post phloem tissues of sink organs also contribute to phloem transport. The protocol described here estimates carbon allocation along the entire path from initial carbon fixation to delivery to sink organs after a labeling pulse: [14C]CO2 is photoassimilated in source leaves and loading and transport of the 14C label to heterotrophic sink organs (roots) is quantified by scintillation counting. This method is flexible and can be adapted to quantify long-distance transport in many plant species.

AB - Phloem loading and long-distance transport of photoassimilate from source leaves to sink organs are essential physiological processes that contribute to plant growth and yield. At a minimum, three steps are involved: phloem loading in source organs, transport along the phloem path, and phloem unloading in sink organs. Each of these can have variable rates contingent on the physiological state of the plant, and thereby influence the overall transport rate. In addition to these phloem transport steps, rates of photosynthesis and photosynthate movement in the pre-phloem path, as well as photosynthate utilization in post phloem tissues of sink organs also contribute to phloem transport. The protocol described here estimates carbon allocation along the entire path from initial carbon fixation to delivery to sink organs after a labeling pulse: [14C]CO2 is photoassimilated in source leaves and loading and transport of the 14C label to heterotrophic sink organs (roots) is quantified by scintillation counting. This method is flexible and can be adapted to quantify long-distance transport in many plant species.

KW - C labeling

KW - Carbon allocation

KW - Phloem transport

KW - Photoassimilate partitioning

KW - Photosynthetic labeling

KW - Source-sink relations

KW - Sugar transport

UR - http://www.scopus.com/inward/record.url?scp=85067461574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067461574&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9562-2_19

DO - 10.1007/978-1-4939-9562-2_19

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 223

EP - 233

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -