Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [14C]CO2

Umesh P. Yadav; Mearaj A. Shaikh; John Evers; Kamesh C. Regmi; Roberto A. Gaxiola; Brian G. Ayre

doi:10.1007/978-1-4939-9562-2_19

Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [¹⁴C]CO₂

Umesh P. Yadav, Mearaj A. Shaikh, John Evers, Kamesh C. Regmi, Roberto A. Gaxiola, Brian G. Ayre

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Phloem loading and long-distance transport of photoassimilate from source leaves to sink organs are essential physiological processes that contribute to plant growth and yield. At a minimum, three steps are involved: phloem loading in source organs, transport along the phloem path, and phloem unloading in sink organs. Each of these can have variable rates contingent on the physiological state of the plant, and thereby influence the overall transport rate. In addition to these phloem transport steps, rates of photosynthesis and photosynthate movement in the pre-phloem path, as well as photosynthate utilization in post phloem tissues of sink organs also contribute to phloem transport. The protocol described here estimates carbon allocation along the entire path from initial carbon fixation to delivery to sink organs after a labeling pulse: [¹⁴C]CO₂ is photoassimilated in source leaves and loading and transport of the ¹⁴C label to heterotrophic sink organs (roots) is quantified by scintillation counting. This method is flexible and can be adapted to quantify long-distance transport in many plant species.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	223-233
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-9562-2_19
State	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2014
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

C labeling
Carbon allocation
Phloem transport
Photoassimilate partitioning
Photosynthetic labeling
Source-sink relations
Sugar transport

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-9562-2_19

Cite this

Yadav, U. P., Shaikh, M. A., Evers, J., Regmi, K. C., Gaxiola, R. A., & Ayre, B. G. (2019). Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [¹⁴C]CO₂ In Methods in Molecular Biology (pp. 223-233). (Methods in Molecular Biology; Vol. 2014). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9562-2_19

Assessing Long-Distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassimilated [¹⁴C]CO₂ . / Yadav, Umesh P.; Shaikh, Mearaj A.; Evers, John et al.
Methods in Molecular Biology. Humana Press Inc., 2019. p. 223-233 (Methods in Molecular Biology; Vol. 2014).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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abstract = "Phloem loading and long-distance transport of photoassimilate from source leaves to sink organs are essential physiological processes that contribute to plant growth and yield. At a minimum, three steps are involved: phloem loading in source organs, transport along the phloem path, and phloem unloading in sink organs. Each of these can have variable rates contingent on the physiological state of the plant, and thereby influence the overall transport rate. In addition to these phloem transport steps, rates of photosynthesis and photosynthate movement in the pre-phloem path, as well as photosynthate utilization in post phloem tissues of sink organs also contribute to phloem transport. The protocol described here estimates carbon allocation along the entire path from initial carbon fixation to delivery to sink organs after a labeling pulse: [14C]CO2 is photoassimilated in source leaves and loading and transport of the 14C label to heterotrophic sink organs (roots) is quantified by scintillation counting. This method is flexible and can be adapted to quantify long-distance transport in many plant species.",

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note = "Funding Information: Work on phloem loading and long-distance transport in B.G. Ayre{\textquoteright}s laboratory is/was supported by the National Science Foundation grants 0344088, 0922546, 1121819, and 1558012. The authors thank Dr. Robert Turgeon for helpful discussion. Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",

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