TY - JOUR
T1 - Assessing dehydroepiandrosterone in saliva
T2 - A simple radioimmunoassay for use in studies of children, adolescents and adults
AU - Granger, Douglas A.
AU - Schwartz, Eve B.
AU - Booth, Alan
AU - Curran, Mary
AU - Zakaria, Dena
N1 - Funding Information:
Thanks are due to Ruth Merritt, Jodi Heaton, Jyotika Mirchandani, Virginia Lucas, and the staff of the Penn State Behavioral Endocrinology Laboratory, as well as the staff of the Penn State Family Relations Project (Ann Crouter and Susan McHale, PIs) for their assistance with various aspects of this project. The studies were supported in part by the Pennsylvania State University Behavioral Endocrinology Laboratory, HYPERLINK http://bbh.hhdev.psu.edu/labs/bel/bel.html, and the W.T. Grant Foundation. We gratefully acknowledge the contribution of reagents and materials by Diagnostic Systems Laboratories (Webster, TX).
PY - 1999/7
Y1 - 1999/7
N2 - While salivary assays for some hormones are widely used, the availability of assays for salivary DHEA is limited. By adapting a commercially available radioimmunoassay serum kit, we developed a reliable, efficient and sensitive measure of DHEA in saliva that does not require separation or extraction. The minimum detection limit was 4.0 pg/ml. Intra- assay coefficients of variation (CV%) were on average 4.05, and inter-assay CVs averaged 9.70. Method accuracy, determined by spike recovery, and linearity, determined by serial dilution, averaged 99.55 and 92.03%. Levels in matched serum and saliva samples showed strong linear relationships for adult males and females. Specific guidelines are developed for sample collection, storage, and preparation procedures. Reference ranges for salivary DHEA levels are provided for 64 children ages 8-11, 96 adolescents ages 12-17 and 48 adults ages 30-45. Salivary DHEA levels are shown to reflect developmental, gender and diurnal differences.
AB - While salivary assays for some hormones are widely used, the availability of assays for salivary DHEA is limited. By adapting a commercially available radioimmunoassay serum kit, we developed a reliable, efficient and sensitive measure of DHEA in saliva that does not require separation or extraction. The minimum detection limit was 4.0 pg/ml. Intra- assay coefficients of variation (CV%) were on average 4.05, and inter-assay CVs averaged 9.70. Method accuracy, determined by spike recovery, and linearity, determined by serial dilution, averaged 99.55 and 92.03%. Levels in matched serum and saliva samples showed strong linear relationships for adult males and females. Specific guidelines are developed for sample collection, storage, and preparation procedures. Reference ranges for salivary DHEA levels are provided for 64 children ages 8-11, 96 adolescents ages 12-17 and 48 adults ages 30-45. Salivary DHEA levels are shown to reflect developmental, gender and diurnal differences.
KW - DHEA
KW - Gender and developmental differences
KW - Radioimmunoassay
KW - Saliva
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U2 - 10.1016/S0306-4530(99)00013-X
DO - 10.1016/S0306-4530(99)00013-X
M3 - Article
C2 - 10378242
AN - SCOPUS:0033067170
SN - 0306-4530
VL - 24
SP - 567
EP - 579
JO - Psychoneuroendocrinology
JF - Psychoneuroendocrinology
IS - 5
ER -