Abstract
This study demonstrates that the glycoprotein of vesicular stomatitis virus clusters in the plasma membrane of infected Chinese hamster lung cells during morphogenesis and suggests that viral nucleocapsids are required for this clustering. A mutant virus (ts E-1) which is temperature sensitive for the synthesis of viral nucleocapsids but not viral membrane proteins was used. The surface distribution of the viral glycoprotein in cells infected by this virus was determined by a specific indirect immunoferritin stain. Early in infection at permissive temperatures, the glycoprotein was randomly distributed on membrane ghosts. Later, clusters of ferritin the size and shape of virus particles were seen. In contrast, ghosts prepared from virus-infected cells maintained at a restrictive temperature always had a random distribution of viral glycoprotein.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1047-1055 |
| Number of pages | 9 |
| Journal | Journal of Virology |
| Volume | 44 |
| Issue number | 3 |
| State | Published - 1982 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Assembly of vesicular stomatitis virus : Distribution of the glycoprotein on the surface of infected cells. / Jacobs, Bertram; Penhoet, E. E.
In: Journal of Virology, Vol. 44, No. 3, 1982, p. 1047-1055.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assembly of vesicular stomatitis virus
T2 - Distribution of the glycoprotein on the surface of infected cells
AU - Jacobs, Bertram
AU - Penhoet, E. E.
PY - 1982
Y1 - 1982
N2 - This study demonstrates that the glycoprotein of vesicular stomatitis virus clusters in the plasma membrane of infected Chinese hamster lung cells during morphogenesis and suggests that viral nucleocapsids are required for this clustering. A mutant virus (ts E-1) which is temperature sensitive for the synthesis of viral nucleocapsids but not viral membrane proteins was used. The surface distribution of the viral glycoprotein in cells infected by this virus was determined by a specific indirect immunoferritin stain. Early in infection at permissive temperatures, the glycoprotein was randomly distributed on membrane ghosts. Later, clusters of ferritin the size and shape of virus particles were seen. In contrast, ghosts prepared from virus-infected cells maintained at a restrictive temperature always had a random distribution of viral glycoprotein.
AB - This study demonstrates that the glycoprotein of vesicular stomatitis virus clusters in the plasma membrane of infected Chinese hamster lung cells during morphogenesis and suggests that viral nucleocapsids are required for this clustering. A mutant virus (ts E-1) which is temperature sensitive for the synthesis of viral nucleocapsids but not viral membrane proteins was used. The surface distribution of the viral glycoprotein in cells infected by this virus was determined by a specific indirect immunoferritin stain. Early in infection at permissive temperatures, the glycoprotein was randomly distributed on membrane ghosts. Later, clusters of ferritin the size and shape of virus particles were seen. In contrast, ghosts prepared from virus-infected cells maintained at a restrictive temperature always had a random distribution of viral glycoprotein.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020466597&partnerID=8YFLogxK
M3 - Article
C2 - 6294321
AN - SCOPUS:0020466597
VL - 44
SP - 1047
EP - 1055
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 3
ER -