Assembly of vesicular stomatitis virus: Distribution of the glycoprotein on the surface of infected cells

Bertram Jacobs, E. E. Penhoet

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study demonstrates that the glycoprotein of vesicular stomatitis virus clusters in the plasma membrane of infected Chinese hamster lung cells during morphogenesis and suggests that viral nucleocapsids are required for this clustering. A mutant virus (ts E-1) which is temperature sensitive for the synthesis of viral nucleocapsids but not viral membrane proteins was used. The surface distribution of the viral glycoprotein in cells infected by this virus was determined by a specific indirect immunoferritin stain. Early in infection at permissive temperatures, the glycoprotein was randomly distributed on membrane ghosts. Later, clusters of ferritin the size and shape of virus particles were seen. In contrast, ghosts prepared from virus-infected cells maintained at a restrictive temperature always had a random distribution of viral glycoprotein.

Original languageEnglish (US)
Pages (from-to)1047-1055
Number of pages9
JournalJournal of Virology
Volume44
Issue number3
StatePublished - 1982
Externally publishedYes

Fingerprint

Vesiculovirus
Vesicular Stomatitis
Membrane Glycoproteins
glycoproteins
Glycoproteins
Viruses
Nucleocapsid
nucleocapsid
viruses
Temperature
Viral Matrix Proteins
cells
temperature
viral proteins
Chinese hamsters
ferritin
Ferritins
Cricetulus
Morphogenesis
virion

ASJC Scopus subject areas

  • Immunology

Cite this

Assembly of vesicular stomatitis virus : Distribution of the glycoprotein on the surface of infected cells. / Jacobs, Bertram; Penhoet, E. E.

In: Journal of Virology, Vol. 44, No. 3, 1982, p. 1047-1055.

Research output: Contribution to journalArticle

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