Assembly of TolC, a Structurally Unique and Multifunctional Outer Membrane Protein of Escherichia coli K-12

John Werner, Anne Marie Augustus, Rajeev Misra

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

TolC is a multifunctional outer membrane protein of Escherichia coli that folds into a novel α-β-barrel conformation absent in the other model outer membrane proteins used in assembly studies. The data presented in this work show that the unique folded structure of TolC reflects a unique assembly pathway. During its assembly, the newly translocated nascent TolC monomers are released in the periplasm. Maturation of these nascent monomers, and possibly their oligomerization, in the periplasm precedes their insertion in the outer membrane. The completion of the assembly process is signaled by the development of a characteristic proteinase K-resistant fragment generated by cleavage at a single, periplasmically exposed, protease-sensitive site of the membrane-anchored trimer. None of the assembly steps of TolC is affected by known folding factors, such as SurA, Skp, and lipopolysaccharide, which have profound effects on the assembly of other model trimeric outer membrane proteins. Two assembly-defective TolC mutants were isolated and characterized. One of the mutants (TolCI106N) was defective in the folding of nascent monomers, while the other (TolCS350F) was impaired in steps involving trimerization and membrane insertion of folded monomers.

Original languageEnglish (US)
Pages (from-to)6540-6547
Number of pages8
JournalJournal of Bacteriology
Volume185
Issue number22
DOIs
StatePublished - Nov 2003

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Periplasm
Membrane Proteins
Escherichia coli
Membranes
Endopeptidase K
Lipopolysaccharides
Peptide Hydrolases

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Assembly of TolC, a Structurally Unique and Multifunctional Outer Membrane Protein of Escherichia coli K-12. / Werner, John; Augustus, Anne Marie; Misra, Rajeev.

In: Journal of Bacteriology, Vol. 185, No. 22, 11.2003, p. 6540-6547.

Research output: Contribution to journalArticle

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