Assembly of LamB and OmpF in deep rough lipopolysaccharide mutants of Escherichia coli K-12

M. W. Laird, A. W. Kloser, Rajeev Misra

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Assembly of the OmpF and LamB proteins was kinetically retarded in deep rough lipopolysaccharide mutants of Escherichia coli K-12. OmpF assembly was affected at the step of conversion of metastable trimers to stable trimers, whereas LamB assembly was influenced both at the monomer-to-metastable trimer and metastable-to-stable trimer steps. These assembly defects were reversed in the presence of the sfaA1 and sfaB3 suppressor alleles, which were isolated by using ompF assembly mutants.

Original languageEnglish (US)
Pages (from-to)2259-2264
Number of pages6
JournalJournal of bacteriology
Volume176
Issue number8
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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