Assay development and storage for fluorescence-based lateral flow immunoassay

Ching Wen Hou; Meilin Zhu; Karen Anderson; Uwadiae Obahiagbon; Jennifer Blain Christen

doi:10.1109/LSC.2018.8572244

Assay development and storage for fluorescence-based lateral flow immunoassay

Ching Wen Hou, Meilin Zhu, Karen Anderson, Uwadiae Obahiagbon, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.

Original language	English (US)
Title of host publication	2018 IEEE Life Sciences Conference, LSC 2018
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	150-153
Number of pages	4
ISBN (Electronic)	9781538667095
DOIs	https://doi.org/10.1109/LSC.2018.8572244
State	Published - Dec 10 2018
Event	2018 IEEE Life Sciences Conference, LSC 2018 - Montreal, Canada Duration: Oct 28 2018 → Oct 30 2018

Other

Other	2018 IEEE Life Sciences Conference, LSC 2018
Country	Canada
City	Montreal
Period	10/28/18 → 10/30/18

Keywords

Fluorescence
Immunoassay
Lateral flow assay
Point-of-care
Sensitivity
Storage

ASJC Scopus subject areas

Signal Processing
Medicine (miscellaneous)
Health Informatics
Instrumentation

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Assay development and storage for fluorescence-based lateral flow immunoassay. / Hou, Ching Wen; Zhu, Meilin; Anderson, Karen; Obahiagbon, Uwadiae; Blain Christen, Jennifer.

2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 150-153 8572244.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hou, CW, Zhu, M, Anderson, K, Obahiagbon, U & Blain Christen, J 2018, Assay development and storage for fluorescence-based lateral flow immunoassay. in 2018 IEEE Life Sciences Conference, LSC 2018., 8572244, Institute of Electrical and Electronics Engineers Inc., pp. 150-153, 2018 IEEE Life Sciences Conference, LSC 2018, Montreal, Canada, 10/28/18. https://doi.org/10.1109/LSC.2018.8572244

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title = "Assay development and storage for fluorescence-based lateral flow immunoassay",

abstract = "Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.",

keywords = "Fluorescence, Immunoassay, Lateral flow assay, Point-of-care, Sensitivity, Storage",

author = "Hou, {Ching Wen} and Meilin Zhu and Karen Anderson and Uwadiae Obahiagbon and {Blain Christen}, Jennifer",

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doi = "10.1109/LSC.2018.8572244",

language = "English (US)",

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AU - Hou, Ching Wen

AU - Zhu, Meilin

AU - Anderson, Karen

AU - Obahiagbon, Uwadiae

AU - Blain Christen, Jennifer

PY - 2018/12/10

Y1 - 2018/12/10

N2 - Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.

AB - Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.

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KW - Immunoassay

KW - Lateral flow assay

KW - Point-of-care

KW - Sensitivity

KW - Storage

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PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2018 IEEE Life Sciences Conference, LSC 2018

Y2 - 28 October 2018 through 30 October 2018

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Assay development and storage for fluorescence-based lateral flow immunoassay

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Keywords

ASJC Scopus subject areas

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