Assay development and storage for fluorescence-based lateral flow immunoassay

Ching Wen Hou, Meilin Zhu, Karen Anderson, Uwadiae Obahiagbon, Jennifer Blain Christen

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.

    Original languageEnglish (US)
    Title of host publication2018 IEEE Life Sciences Conference, LSC 2018
    PublisherInstitute of Electrical and Electronics Engineers Inc.
    Pages150-153
    Number of pages4
    ISBN (Electronic)9781538667095
    DOIs
    StatePublished - Dec 10 2018
    Event2018 IEEE Life Sciences Conference, LSC 2018 - Montreal, Canada
    Duration: Oct 28 2018Oct 30 2018

    Other

    Other2018 IEEE Life Sciences Conference, LSC 2018
    CountryCanada
    CityMontreal
    Period10/28/1810/30/18

    Fingerprint

    Papillomaviridae
    immunoassay
    viruses
    antibodies
    Immunoassay
    Assays
    Fluorescence
    Point-of-Care Systems
    income
    fluorescence
    Nuclear Antigens
    Antibodies
    biomarkers
    washing
    antigens
    Viruses
    Health care
    Uterine Cervical Neoplasms
    Health Care Costs
    health

    Keywords

    • Fluorescence
    • Immunoassay
    • Lateral flow assay
    • Point-of-care
    • Sensitivity
    • Storage

    ASJC Scopus subject areas

    • Signal Processing
    • Medicine (miscellaneous)
    • Health Informatics
    • Instrumentation

    Cite this

    Hou, C. W., Zhu, M., Anderson, K., Obahiagbon, U., & Blain Christen, J. (2018). Assay development and storage for fluorescence-based lateral flow immunoassay. In 2018 IEEE Life Sciences Conference, LSC 2018 (pp. 150-153). [8572244] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/LSC.2018.8572244

    Assay development and storage for fluorescence-based lateral flow immunoassay. / Hou, Ching Wen; Zhu, Meilin; Anderson, Karen; Obahiagbon, Uwadiae; Blain Christen, Jennifer.

    2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 150-153 8572244.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Hou, CW, Zhu, M, Anderson, K, Obahiagbon, U & Blain Christen, J 2018, Assay development and storage for fluorescence-based lateral flow immunoassay. in 2018 IEEE Life Sciences Conference, LSC 2018., 8572244, Institute of Electrical and Electronics Engineers Inc., pp. 150-153, 2018 IEEE Life Sciences Conference, LSC 2018, Montreal, Canada, 10/28/18. https://doi.org/10.1109/LSC.2018.8572244
    Hou CW, Zhu M, Anderson K, Obahiagbon U, Blain Christen J. Assay development and storage for fluorescence-based lateral flow immunoassay. In 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc. 2018. p. 150-153. 8572244 https://doi.org/10.1109/LSC.2018.8572244
    Hou, Ching Wen ; Zhu, Meilin ; Anderson, Karen ; Obahiagbon, Uwadiae ; Blain Christen, Jennifer. / Assay development and storage for fluorescence-based lateral flow immunoassay. 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 150-153
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