Abstract
Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.
| Original language | English (US) |
|---|---|
| Title of host publication | 2018 IEEE Life Sciences Conference, LSC 2018 |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 150-153 |
| Number of pages | 4 |
| ISBN (Electronic) | 9781538667095 |
| DOIs | |
| State | Published - Dec 10 2018 |
| Event | 2018 IEEE Life Sciences Conference, LSC 2018 - Montreal, Canada Duration: Oct 28 2018 → Oct 30 2018 |
Other
| Other | 2018 IEEE Life Sciences Conference, LSC 2018 |
|---|---|
| Country | Canada |
| City | Montreal |
| Period | 10/28/18 → 10/30/18 |
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Keywords
- Fluorescence
- Immunoassay
- Lateral flow assay
- Point-of-care
- Sensitivity
- Storage
ASJC Scopus subject areas
- Signal Processing
- Medicine (miscellaneous)
- Health Informatics
- Instrumentation
Cite this
Assay development and storage for fluorescence-based lateral flow immunoassay. / Hou, Ching Wen; Zhu, Meilin; Anderson, Karen; Obahiagbon, Uwadiae; Blain Christen, Jennifer.
2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 150-153 8572244.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Assay development and storage for fluorescence-based lateral flow immunoassay
AU - Hou, Ching Wen
AU - Zhu, Meilin
AU - Anderson, Karen
AU - Obahiagbon, Uwadiae
AU - Blain Christen, Jennifer
PY - 2018/12/10
Y1 - 2018/12/10
N2 - Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.
AB - Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.
KW - Fluorescence
KW - Immunoassay
KW - Lateral flow assay
KW - Point-of-care
KW - Sensitivity
KW - Storage
UR - http://www.scopus.com/inward/record.url?scp=85060185717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060185717&partnerID=8YFLogxK
U2 - 10.1109/LSC.2018.8572244
DO - 10.1109/LSC.2018.8572244
M3 - Conference contribution
SP - 150
EP - 153
BT - 2018 IEEE Life Sciences Conference, LSC 2018
PB - Institute of Electrical and Electronics Engineers Inc.
ER -