Abstract

Point-of-care medical diagnostics can provide efficient, cost-effective medical care, and have the potential to fundamentally change our current approach to global health. There have been substantial efforts in developing lateral flow assays for serologic testing, but most of the existing approaches have limited portability, are expensive, and offer limited analytical sensitivity. In this paper, we demonstrated an assay for the detection of antibodies in plasma to Epstein-Barr Nuclear Antigen-1 (EBNA-1) protein and optimization of the assay including washing and blocking conditions. We also investigated the effect of the storage on the assay strips. Using our optimized conditions, we were able to detect anti-Human Papilloma Virus (HPV)-16 E7 antibodies after three weeks of storage. Our goal is to adapt this system to detect HPV biomarkers for cervical cancers in low and middle-income countries.

Original languageEnglish (US)
Title of host publication2018 IEEE Life Sciences Conference, LSC 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages150-153
Number of pages4
ISBN (Electronic)9781538667095
DOIs
StatePublished - Dec 10 2018
Event2018 IEEE Life Sciences Conference, LSC 2018 - Montreal, Canada
Duration: Oct 28 2018Oct 30 2018

Other

Other2018 IEEE Life Sciences Conference, LSC 2018
CountryCanada
CityMontreal
Period10/28/1810/30/18

Fingerprint

Papillomaviridae
immunoassay
viruses
antibodies
Immunoassay
Assays
Fluorescence
Point-of-Care Systems
income
fluorescence
Nuclear Antigens
Antibodies
biomarkers
washing
antigens
Viruses
Health care
Uterine Cervical Neoplasms
Health Care Costs
health

Keywords

  • Fluorescence
  • Immunoassay
  • Lateral flow assay
  • Point-of-care
  • Sensitivity
  • Storage

ASJC Scopus subject areas

  • Signal Processing
  • Medicine (miscellaneous)
  • Health Informatics
  • Instrumentation

Cite this

Hou, C. W., Zhu, M., Anderson, K., Obahiagbon, U., & Blain Christen, J. (2018). Assay development and storage for fluorescence-based lateral flow immunoassay. In 2018 IEEE Life Sciences Conference, LSC 2018 (pp. 150-153). [8572244] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/LSC.2018.8572244

Assay development and storage for fluorescence-based lateral flow immunoassay. / Hou, Ching Wen; Zhu, Meilin; Anderson, Karen; Obahiagbon, Uwadiae; Blain Christen, Jennifer.

2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 150-153 8572244.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hou, CW, Zhu, M, Anderson, K, Obahiagbon, U & Blain Christen, J 2018, Assay development and storage for fluorescence-based lateral flow immunoassay. in 2018 IEEE Life Sciences Conference, LSC 2018., 8572244, Institute of Electrical and Electronics Engineers Inc., pp. 150-153, 2018 IEEE Life Sciences Conference, LSC 2018, Montreal, Canada, 10/28/18. https://doi.org/10.1109/LSC.2018.8572244
Hou CW, Zhu M, Anderson K, Obahiagbon U, Blain Christen J. Assay development and storage for fluorescence-based lateral flow immunoassay. In 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc. 2018. p. 150-153. 8572244 https://doi.org/10.1109/LSC.2018.8572244
Hou, Ching Wen ; Zhu, Meilin ; Anderson, Karen ; Obahiagbon, Uwadiae ; Blain Christen, Jennifer. / Assay development and storage for fluorescence-based lateral flow immunoassay. 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 150-153
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