Arrest of membrane fusion events in mast cells by quick-freezing

D. E. Chandler, J. E. Heuser

Research output: Contribution to journalArticle

183 Scopus citations

Abstract

We have used quick-freezing and freeze-fracture to study early stages of exocytosis in rat peritoneal mast cells. Mast cells briefly stimulated with 48/80 (a synthetic polycation and well-known histamine-releasing agent) at 22°C displayed single, narrow-necked pores (some as small as 0.05 jum in diameter) joining single granules with the plasma membrane. Pores that had become as large as 0.1 jum in diameter were clearly etchable and thus represented aqueous channels connecting the granule interior with the extracellular space. Granules exhibiting pores usually did not have wide areas of contact with the plasma membrane, and clearings of intramembrane particles, seen in chemically fixed mast cells undergoing exocytosis, were not present on either plasma or granule membranes. Fusion of interior granules later in the secretory process also appeared to involve pores; granules were often joined by one pore or a group of 2-4 pores. Also found were groups of extremely small, etchable pores on granule membranes that may represent the earliest aqueous communication between fusing granules.

Original languageEnglish (US)
Pages (from-to)666-674
Number of pages9
JournalJournal of Cell Biology
Volume86
Issue number2
DOIs
StatePublished - Aug 1 1980

ASJC Scopus subject areas

  • Cell Biology

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