Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populations

David Brafman, Shu Chien, Karl Willert

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

During the development of an organism, cells are exposed to a myriad of signals, structural components and scaffolds, which collectively make up the cellular microenvironment. The majority of current developmental biology studies examine the effect of individual or small subsets of molecules and parameters on cellular behavior, and they consequently fail to explore the complexity of factors to which cells are exposed. Here we describe a technology, referred to as arrayed cellular microenvironments (ACMEs), that allows for a high-throughput examination of the effects of multiple extracellular components in a combinatorial manner on any cell type of interest. We will specifically focus on the application of this technology to human pluripotent stem cells (hPSCs), a population of cells with tremendous therapeutic potential, and one for which growth and differentiation conditions are poorly characterized and far from defined and optimized. A standard ACME screen uses the technologies previously applied to the manufacture and analysis of DNA microarrays, requires standard cell-culture facilities and can be performed from beginning to end within 5-10 days.

Original languageEnglish (US)
Pages (from-to)703-717
Number of pages15
JournalNature Protocols
Volume7
Issue number4
DOIs
StatePublished - Apr 2012
Externally publishedYes

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Cellular Microenvironment
Cells
Technology
Population
Microarrays
Stem cells
Cell culture
Scaffolds
Developmental Biology
Pluripotent Stem Cells
Oligonucleotide Array Sequence Analysis
Throughput
Molecules
Cell Culture Techniques
DNA
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populations. / Brafman, David; Chien, Shu; Willert, Karl.

In: Nature Protocols, Vol. 7, No. 4, 04.2012, p. 703-717.

Research output: Contribution to journalArticle

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