Archaeal populations in biological soil crusts from arid lands in North America

Tanya Soule; Ian J. Anderson; Shannon L. Johnson; Scott T. Bates; Ferran Garcia-Pichel

doi:10.1016/j.soilbio.2009.07.023

Archaeal populations in biological soil crusts from arid lands in North America

Tanya Soule, Ian J. Anderson, Shannon L. Johnson, Scott T. Bates, Ferran Garcia-Pichel

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Archaea are common and abundant members of biological soil crust communities across large-scale biogeographic provinces of arid North America. Regardless of microbial community development, archaeal populations averaged 2 × 10⁷ 16S rRNA gene copies per gram of soil, representing around 5% of the prokaryotic (total calculated bacterial and archaeal) numbers assessed by quantitative-PCR. In contrast, archaeal diversity, determined by denaturing gradient gel electrophoresis fingerprinting and clone libraries of 16S rRNA genes, was very restricted. Only six different phylotypes (all Crenarchaea) were detected, three of which were very dominant. Some phylotypes were widespread, while others were typical of Southern desert areas.

Original language	English (US)
Pages (from-to)	2069-2074
Number of pages	6
Journal	Soil Biology and Biochemistry
Volume	41
Issue number	10
DOIs	https://doi.org/10.1016/j.soilbio.2009.07.023
State	Published - Oct 2009

Keywords

16S rRNA
Archaea
Biological soil crust
Desert
Mixed community

ASJC Scopus subject areas

Microbiology
Soil Science

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10.1016/j.soilbio.2009.07.023

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title = "Archaeal populations in biological soil crusts from arid lands in North America",

abstract = "Archaea are common and abundant members of biological soil crust communities across large-scale biogeographic provinces of arid North America. Regardless of microbial community development, archaeal populations averaged 2 × 107 16S rRNA gene copies per gram of soil, representing around 5% of the prokaryotic (total calculated bacterial and archaeal) numbers assessed by quantitative-PCR. In contrast, archaeal diversity, determined by denaturing gradient gel electrophoresis fingerprinting and clone libraries of 16S rRNA genes, was very restricted. Only six different phylotypes (all Crenarchaea) were detected, three of which were very dominant. Some phylotypes were widespread, while others were typical of Southern desert areas.",

keywords = "16S rRNA, Archaea, Biological soil crust, Desert, Mixed community",

author = "Tanya Soule and Anderson, {Ian J.} and Johnson, {Shannon L.} and Bates, {Scott T.} and Ferran Garcia-Pichel",

note = "Funding Information: We thank Moria Nagy and S.N.G. Reddy for sharing their initial experiences with crust Archaea. The staff of the Sevilleta and Jornada LTER sites, as well as the National Park Service (Canyonlands, N.P. and Organ Pipe N.M.), are gratefully acknowledged for providing sampling permits, guidance, and hospitality. Finally, we thank Dr. Scott Bingham for assistance with qPCR and for facilitating sequencing. This research was funded by an NSF grant from the Biodiversity Surveys and Inventories Program and by a USDA grant from the Soil Processes Program.",

year = "2009",

month = oct,

doi = "10.1016/j.soilbio.2009.07.023",

language = "English (US)",

volume = "41",

pages = "2069--2074",

journal = "Soil Biology and Biochemistry",

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AU - Soule, Tanya

AU - Anderson, Ian J.

AU - Johnson, Shannon L.

AU - Bates, Scott T.

AU - Garcia-Pichel, Ferran

N1 - Funding Information: We thank Moria Nagy and S.N.G. Reddy for sharing their initial experiences with crust Archaea. The staff of the Sevilleta and Jornada LTER sites, as well as the National Park Service (Canyonlands, N.P. and Organ Pipe N.M.), are gratefully acknowledged for providing sampling permits, guidance, and hospitality. Finally, we thank Dr. Scott Bingham for assistance with qPCR and for facilitating sequencing. This research was funded by an NSF grant from the Biodiversity Surveys and Inventories Program and by a USDA grant from the Soil Processes Program.

PY - 2009/10

Y1 - 2009/10

N2 - Archaea are common and abundant members of biological soil crust communities across large-scale biogeographic provinces of arid North America. Regardless of microbial community development, archaeal populations averaged 2 × 107 16S rRNA gene copies per gram of soil, representing around 5% of the prokaryotic (total calculated bacterial and archaeal) numbers assessed by quantitative-PCR. In contrast, archaeal diversity, determined by denaturing gradient gel electrophoresis fingerprinting and clone libraries of 16S rRNA genes, was very restricted. Only six different phylotypes (all Crenarchaea) were detected, three of which were very dominant. Some phylotypes were widespread, while others were typical of Southern desert areas.

AB - Archaea are common and abundant members of biological soil crust communities across large-scale biogeographic provinces of arid North America. Regardless of microbial community development, archaeal populations averaged 2 × 107 16S rRNA gene copies per gram of soil, representing around 5% of the prokaryotic (total calculated bacterial and archaeal) numbers assessed by quantitative-PCR. In contrast, archaeal diversity, determined by denaturing gradient gel electrophoresis fingerprinting and clone libraries of 16S rRNA genes, was very restricted. Only six different phylotypes (all Crenarchaea) were detected, three of which were very dominant. Some phylotypes were widespread, while others were typical of Southern desert areas.

KW - 16S rRNA

KW - Archaea

KW - Biological soil crust

KW - Desert

KW - Mixed community

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JO - Soil Biology and Biochemistry

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Archaeal populations in biological soil crusts from arid lands in North America

Abstract

Keywords

ASJC Scopus subject areas

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