Arabidopsis sodium dependent and independent phenotypes triggered by H+-PPase up-regulation are SOS1 dependent

Soledad F. Undurraga, Mirella P. Santos, Julio Paez-Valencia, Haibing Yang, Peter K. Hepler, Arnoldo R. Facanha, Kendal D. Hirschi, Roberto Gaxiola

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Coordinate regulation of transporters at both the plasma membrane and vacuole contribute to plant cell's ability to adapt to a changing environment and play a key role in the maintenance of the chemiosmotic circuits required for cellular growth. The plasma membrane (PM) Na +/H + antiporter (SOS1) is involved in salt tolerance, presumably in sodium extrusion; the vacuolar type I H +-PPase AVP1 is involved in vacuolar sodium sequestration, but its overexpression has also been shown to alter the abundance and activity of the PM H +-ATPase. Here we investigate the relationship between these transporters utilizing loss-of-function mutants of SOS1 (sos1) and increased expression of AVP1 (AVP1OX). Heightened expression of AVP1 enhances pyrophosphate-dependent proton pump activity, salt tolerance, ion vacuolar sequestration, K + uptake capacity, root hair development, osmotic responses, and PM ATPase hydrolytic and proton pumping activities. In sos1 lines overexpressing AVP1, these phenotypes are negatively affected demonstrating that sos1 is epistatic to AVP1. Enhanced AVP1 protein levels require SOS1 and this regulation appears to be post-translational.

Original languageEnglish (US)
Pages (from-to)96-105
Number of pages10
JournalPlant Science
Volume183
DOIs
StatePublished - Feb 2012

Fingerprint

Inorganic Pyrophosphatase
Arabidopsis
Up-Regulation
plasma membrane
Sodium
Cell Membrane
sodium
Salt-Tolerance
Phenotype
phenotype
salt tolerance
transporters
Proton Pumps
Sodium-Hydrogen Antiporter
proton pump
H-transporting ATP synthase
Proton-Translocating ATPases
pyrophosphates
Plant Cells
root hairs

Keywords

  • Arabidopsis thaliana
  • AVP1
  • Epistasis
  • Proton pump activity
  • SOS1

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

Cite this

Arabidopsis sodium dependent and independent phenotypes triggered by H+-PPase up-regulation are SOS1 dependent. / Undurraga, Soledad F.; Santos, Mirella P.; Paez-Valencia, Julio; Yang, Haibing; Hepler, Peter K.; Facanha, Arnoldo R.; Hirschi, Kendal D.; Gaxiola, Roberto.

In: Plant Science, Vol. 183, 02.2012, p. 96-105.

Research output: Contribution to journalArticle

Undurraga, SF, Santos, MP, Paez-Valencia, J, Yang, H, Hepler, PK, Facanha, AR, Hirschi, KD & Gaxiola, R 2012, 'Arabidopsis sodium dependent and independent phenotypes triggered by H+-PPase up-regulation are SOS1 dependent', Plant Science, vol. 183, pp. 96-105. https://doi.org/10.1016/j.plantsci.2011.11.011
Undurraga, Soledad F. ; Santos, Mirella P. ; Paez-Valencia, Julio ; Yang, Haibing ; Hepler, Peter K. ; Facanha, Arnoldo R. ; Hirschi, Kendal D. ; Gaxiola, Roberto. / Arabidopsis sodium dependent and independent phenotypes triggered by H+-PPase up-regulation are SOS1 dependent. In: Plant Science. 2012 ; Vol. 183. pp. 96-105.
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