Aptamers Switch on Fluorescence of Triphenylmethane Dyes

Jeremy Babendure, Stephen R. Adams, Roger Y. Tsien

Research output: Contribution to journalArticle

284 Citations (Scopus)

Abstract

MG and SRB aptamers, which are short RNA sequences originally selected only for binding to malachite green or sulforhodamine B, can greatly enhance the fluorescence of normally nonfluorescent triphenylmethane dyes. MG aptamer enhances the quantum yields of malachite green (MG) and a novel rigidized derivative, indolinyl malachite green (IMG) by >2000-fold. SRB aptamer brightens patent blue V and VF by >90-fold. These enhancements are specific because MG aptamer has no effect on patent blue dyes and SRB aptamer has little or no effect on MG and IMG. Such sequence-specific fluorescence labeling of short RNA motifs is a first step toward genetically encodable fusion tags for imaging selected RNAs in vitro and in cells.

Original languageEnglish (US)
Pages (from-to)14716-14717
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number48
DOIs
StatePublished - Dec 3 2003
Externally publishedYes

Fingerprint

RNA
Coloring Agents
Dyes
Fluorescence
Switches
Quantum yield
Labeling
Fusion reactions
lissamine rhodamine B
Derivatives
Imaging techniques
Nucleotide Motifs
triphenylmethane
malachite green

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Aptamers Switch on Fluorescence of Triphenylmethane Dyes. / Babendure, Jeremy; Adams, Stephen R.; Tsien, Roger Y.

In: Journal of the American Chemical Society, Vol. 125, No. 48, 03.12.2003, p. 14716-14717.

Research output: Contribution to journalArticle

Babendure, Jeremy ; Adams, Stephen R. ; Tsien, Roger Y. / Aptamers Switch on Fluorescence of Triphenylmethane Dyes. In: Journal of the American Chemical Society. 2003 ; Vol. 125, No. 48. pp. 14716-14717.
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