Approaches to time-resolved diffraction using an XFEL

    Research output: Contribution to journalArticle

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    Abstract

    We describe several schemes for time-resolved imaging of molecular motion using a freeelectron laser (XFEL), in response to the many challenges and opportunities which XFEL radiation has created for accurate time-resolved measurement of structure. For pump- probe experiments using crystals, the problem of recording full Bragg reflections (not partials) in each shot arises. Two solutions, the use of the large bandwith which necesarily results from using attosecond pulses, and the use the coherent convergent beam mode are suggested. We also show that with attosecond recording times shorter than the temporal coherence time, Bragg reflections excited by different wavelengths from different reflections can interfere, providing structure factor phase information. For slower processes, a mixing jet sample-delivery device is described to allow snapshot solution scattering during molecular reactions on the microsecond scale. For optically excited membrane proteins, we suggest the use of the lipid cubic phase sample delivery device operating at atmospheric pressure. The use of two-color and split-and-delay schemes is suggested for improved accuracy in the Monte-Carlo method of serial femtosecond crystallography (SFX).

    Original languageEnglish (US)
    Pages (from-to)429-438
    Number of pages10
    JournalFaraday Discussions
    Volume171
    DOIs
    StatePublished - Dec 1 2014

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    Diffraction
    delivery
    recording
    diffraction
    Crystallography
    Time measurement
    shot
    Atmospheric pressure
    crystallography
    Monte Carlo method
    lipids
    atmospheric pressure
    Membrane Proteins
    Monte Carlo methods
    time measurement
    Pumps
    Scattering
    pumps
    membranes
    Color

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry

    Cite this

    Approaches to time-resolved diffraction using an XFEL. / Spence, John.

    In: Faraday Discussions, Vol. 171, 01.12.2014, p. 429-438.

    Research output: Contribution to journalArticle

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