Applying Electric Cell-substrate Impedance Sensing (ECIS) to study cell adhesion and cell spreading of an individual cell

This work presents a novel application of Electric Cell-substrate Impedance Sensing (ECIS) to study cell adhesion and cell spreading. Specifically, the measurement of the cell substrate separation (h) and the projected area (Acell) of the cell from the ECIS technique was used to discriminate two individual adherent cell populations that were present during the initial cell adhesion and cell spreading processes. Experimental results from ECIS measurement demonstrated good agreement with those obtained from conventional methods.