Applications and stability of a thermoelectric enzyme sensor

Michael J. Muehlbauer, Eric J. Guilbeau, Bruce C. Towe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Remarkable room-temperature sensitivity and stability have been achieved with thermoelectric enzyme sensors that detect the heat generated by various substrates reacting selectively on their enzyme-immobilized surfaces. Thin-film thermopiles together with cross-linked membranes of glucose oxidase and catalase have been employed to measure a linear voltage response to glucose [30 nV/ (mg/dl)] for concentrations ranging from 16 to 280 mg/dl in oxygen-saturated solutions. 90% response time values are typically less than six seconds. One sensor stored at room temperature and operated intermittently did not significantly change its response to glucose over the course of five days, and ultimately failed after nine days. Another sensor stored at 37°C exhibited a daily decrease in sensitivity and failed after five days. Four sensors operated continuously at room temperature showed an average normalized decline in response to glucose of 0.0087 ± 0.0005 h-1 and operating times ranging between 30 and 78 h. Through the use of alternative enzymes, other sensors have been made that respond to either hydrogen peroxide or urea. The use of an alternative sensing mode in which the rate of voltage change is measured following a step change in concentration appears to extend the upper range of linear response to glucose.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalSensors and Actuators: B. Chemical
Volume2
Issue number3
DOIs
StatePublished - 1990

Fingerprint

Enzyme sensors
glucose
Glucose
enzymes
sensors
Sensors
Thermopiles
room temperature
Immobilized Enzymes
Glucose Oxidase
Glucose oxidase
Electric potential
thermopiles
catalase
Hydrogen peroxide
Urea
Catalase
Temperature
Hydrogen Peroxide
oxidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

Cite this

Applications and stability of a thermoelectric enzyme sensor. / Muehlbauer, Michael J.; Guilbeau, Eric J.; Towe, Bruce C.

In: Sensors and Actuators: B. Chemical, Vol. 2, No. 3, 1990, p. 223-232.

Research output: Contribution to journalArticle

Muehlbauer, Michael J. ; Guilbeau, Eric J. ; Towe, Bruce C. / Applications and stability of a thermoelectric enzyme sensor. In: Sensors and Actuators: B. Chemical. 1990 ; Vol. 2, No. 3. pp. 223-232.
@article{699494ec530b445b994fa4446e1d50ab,
title = "Applications and stability of a thermoelectric enzyme sensor",
abstract = "Remarkable room-temperature sensitivity and stability have been achieved with thermoelectric enzyme sensors that detect the heat generated by various substrates reacting selectively on their enzyme-immobilized surfaces. Thin-film thermopiles together with cross-linked membranes of glucose oxidase and catalase have been employed to measure a linear voltage response to glucose [30 nV/ (mg/dl)] for concentrations ranging from 16 to 280 mg/dl in oxygen-saturated solutions. 90{\%} response time values are typically less than six seconds. One sensor stored at room temperature and operated intermittently did not significantly change its response to glucose over the course of five days, and ultimately failed after nine days. Another sensor stored at 37°C exhibited a daily decrease in sensitivity and failed after five days. Four sensors operated continuously at room temperature showed an average normalized decline in response to glucose of 0.0087 ± 0.0005 h-1 and operating times ranging between 30 and 78 h. Through the use of alternative enzymes, other sensors have been made that respond to either hydrogen peroxide or urea. The use of an alternative sensing mode in which the rate of voltage change is measured following a step change in concentration appears to extend the upper range of linear response to glucose.",
author = "Muehlbauer, {Michael J.} and Guilbeau, {Eric J.} and Towe, {Bruce C.}",
year = "1990",
doi = "10.1016/0925-4005(90)85009-N",
language = "English (US)",
volume = "2",
pages = "223--232",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Applications and stability of a thermoelectric enzyme sensor

AU - Muehlbauer, Michael J.

AU - Guilbeau, Eric J.

AU - Towe, Bruce C.

PY - 1990

Y1 - 1990

N2 - Remarkable room-temperature sensitivity and stability have been achieved with thermoelectric enzyme sensors that detect the heat generated by various substrates reacting selectively on their enzyme-immobilized surfaces. Thin-film thermopiles together with cross-linked membranes of glucose oxidase and catalase have been employed to measure a linear voltage response to glucose [30 nV/ (mg/dl)] for concentrations ranging from 16 to 280 mg/dl in oxygen-saturated solutions. 90% response time values are typically less than six seconds. One sensor stored at room temperature and operated intermittently did not significantly change its response to glucose over the course of five days, and ultimately failed after nine days. Another sensor stored at 37°C exhibited a daily decrease in sensitivity and failed after five days. Four sensors operated continuously at room temperature showed an average normalized decline in response to glucose of 0.0087 ± 0.0005 h-1 and operating times ranging between 30 and 78 h. Through the use of alternative enzymes, other sensors have been made that respond to either hydrogen peroxide or urea. The use of an alternative sensing mode in which the rate of voltage change is measured following a step change in concentration appears to extend the upper range of linear response to glucose.

AB - Remarkable room-temperature sensitivity and stability have been achieved with thermoelectric enzyme sensors that detect the heat generated by various substrates reacting selectively on their enzyme-immobilized surfaces. Thin-film thermopiles together with cross-linked membranes of glucose oxidase and catalase have been employed to measure a linear voltage response to glucose [30 nV/ (mg/dl)] for concentrations ranging from 16 to 280 mg/dl in oxygen-saturated solutions. 90% response time values are typically less than six seconds. One sensor stored at room temperature and operated intermittently did not significantly change its response to glucose over the course of five days, and ultimately failed after nine days. Another sensor stored at 37°C exhibited a daily decrease in sensitivity and failed after five days. Four sensors operated continuously at room temperature showed an average normalized decline in response to glucose of 0.0087 ± 0.0005 h-1 and operating times ranging between 30 and 78 h. Through the use of alternative enzymes, other sensors have been made that respond to either hydrogen peroxide or urea. The use of an alternative sensing mode in which the rate of voltage change is measured following a step change in concentration appears to extend the upper range of linear response to glucose.

UR - http://www.scopus.com/inward/record.url?scp=0025476062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025476062&partnerID=8YFLogxK

U2 - 10.1016/0925-4005(90)85009-N

DO - 10.1016/0925-4005(90)85009-N

M3 - Article

AN - SCOPUS:0025476062

VL - 2

SP - 223

EP - 232

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 3

ER -