Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers

Benjamin A. Katchman, Joseph T. Smith, Uwadiae Obahiagbon, Sailaja Kesiraju, Yong Kyun Lee, Barry O'Brien, Korhan Kaftanoglu, Jennifer Blain Christen, Karen Anderson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Point-of-care molecular diagnostics can provide efficient and cost-effective medical care, and they have the potential to fundamentally change our approach to global health. However, most existing approaches are not scalable to include multiple biomarkers. As a solution, we have combined commercial flat panel OLED display technology with protein microarray technology to enable high-density fluorescent, programmable, multiplexed biorecognition in a compact and disposable configuration with clinical-level sensitivity. Our approach leverages advances in commercial display technology to reduce pre-functionalized biosensor substrate costs to pennies per cm 2. Here, we demonstrate quantitative detection of IgG antibodies to multiple viral antigens in patient serum samples with detection limits for human IgG in the 10 pg/mL range. We also demonstrate multiplexed detection of antibodies to the HPV16 proteins E2, E6, and E7, which are circulating biomarkers for cervical as well as head and neck cancers.

Original languageEnglish (US)
Article number29057
JournalScientific Reports
Volume6
DOIs
StatePublished - Jul 4 2016

Fingerprint

Point-of-Care Systems
Tumor Biomarkers
Technology
Immunoglobulin G
Biomarkers
Protein Array Analysis
Molecular Pathology
Viral Antigens
Antibodies
Biosensing Techniques
Head and Neck Neoplasms
Health Care Costs
Limit of Detection
Costs and Cost Analysis
Serum
Proteins

ASJC Scopus subject areas

  • General

Cite this

Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers. / Katchman, Benjamin A.; Smith, Joseph T.; Obahiagbon, Uwadiae; Kesiraju, Sailaja; Lee, Yong Kyun; O'Brien, Barry; Kaftanoglu, Korhan; Blain Christen, Jennifer; Anderson, Karen.

In: Scientific Reports, Vol. 6, 29057, 04.07.2016.

Research output: Contribution to journalArticle

Katchman, Benjamin A. ; Smith, Joseph T. ; Obahiagbon, Uwadiae ; Kesiraju, Sailaja ; Lee, Yong Kyun ; O'Brien, Barry ; Kaftanoglu, Korhan ; Blain Christen, Jennifer ; Anderson, Karen. / Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers. In: Scientific Reports. 2016 ; Vol. 6.
@article{d52084e516214ce587a7454947dfe25e,
title = "Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers",
abstract = "Point-of-care molecular diagnostics can provide efficient and cost-effective medical care, and they have the potential to fundamentally change our approach to global health. However, most existing approaches are not scalable to include multiple biomarkers. As a solution, we have combined commercial flat panel OLED display technology with protein microarray technology to enable high-density fluorescent, programmable, multiplexed biorecognition in a compact and disposable configuration with clinical-level sensitivity. Our approach leverages advances in commercial display technology to reduce pre-functionalized biosensor substrate costs to pennies per cm 2. Here, we demonstrate quantitative detection of IgG antibodies to multiple viral antigens in patient serum samples with detection limits for human IgG in the 10 pg/mL range. We also demonstrate multiplexed detection of antibodies to the HPV16 proteins E2, E6, and E7, which are circulating biomarkers for cervical as well as head and neck cancers.",
author = "Katchman, {Benjamin A.} and Smith, {Joseph T.} and Uwadiae Obahiagbon and Sailaja Kesiraju and Lee, {Yong Kyun} and Barry O'Brien and Korhan Kaftanoglu and {Blain Christen}, Jennifer and Karen Anderson",
year = "2016",
month = "7",
day = "4",
doi = "10.1038/srep29057",
language = "English (US)",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers

AU - Katchman, Benjamin A.

AU - Smith, Joseph T.

AU - Obahiagbon, Uwadiae

AU - Kesiraju, Sailaja

AU - Lee, Yong Kyun

AU - O'Brien, Barry

AU - Kaftanoglu, Korhan

AU - Blain Christen, Jennifer

AU - Anderson, Karen

PY - 2016/7/4

Y1 - 2016/7/4

N2 - Point-of-care molecular diagnostics can provide efficient and cost-effective medical care, and they have the potential to fundamentally change our approach to global health. However, most existing approaches are not scalable to include multiple biomarkers. As a solution, we have combined commercial flat panel OLED display technology with protein microarray technology to enable high-density fluorescent, programmable, multiplexed biorecognition in a compact and disposable configuration with clinical-level sensitivity. Our approach leverages advances in commercial display technology to reduce pre-functionalized biosensor substrate costs to pennies per cm 2. Here, we demonstrate quantitative detection of IgG antibodies to multiple viral antigens in patient serum samples with detection limits for human IgG in the 10 pg/mL range. We also demonstrate multiplexed detection of antibodies to the HPV16 proteins E2, E6, and E7, which are circulating biomarkers for cervical as well as head and neck cancers.

AB - Point-of-care molecular diagnostics can provide efficient and cost-effective medical care, and they have the potential to fundamentally change our approach to global health. However, most existing approaches are not scalable to include multiple biomarkers. As a solution, we have combined commercial flat panel OLED display technology with protein microarray technology to enable high-density fluorescent, programmable, multiplexed biorecognition in a compact and disposable configuration with clinical-level sensitivity. Our approach leverages advances in commercial display technology to reduce pre-functionalized biosensor substrate costs to pennies per cm 2. Here, we demonstrate quantitative detection of IgG antibodies to multiple viral antigens in patient serum samples with detection limits for human IgG in the 10 pg/mL range. We also demonstrate multiplexed detection of antibodies to the HPV16 proteins E2, E6, and E7, which are circulating biomarkers for cervical as well as head and neck cancers.

UR - http://www.scopus.com/inward/record.url?scp=84977266438&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84977266438&partnerID=8YFLogxK

U2 - 10.1038/srep29057

DO - 10.1038/srep29057

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 29057

ER -