Application of electron tomography to fungal ultrastructure studies

Martin F. Hohmann-Marriott, Maho Uchida, Allison M L Van De Meene, Matthew Garret, Brooke E. Hjelm, Shylaja Kokoori, Robert Roberson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Access to structural information at the nanoscale enables fundamental insights into many complex biological systems. The development of the transmission electron microscope (TEM) has vastly increased our understanding of multiple biological systems. However, when attempting to visualize and understand the organizational and functional complexities that are typical of cells and tissues, the standard 2-D analyses that TEM affords often fall short. In recent years, high-resolution electron tomography methods, coupled with advances in specimen preparation and instrumentation and computational speed, have resulted in a revolution in the biological sciences. Electron tomography is analogous to medical computerized axial tomography (CAT-scan imaging) except at a far finer scale. It utilizes the TEM to assemble multiple projections of an object which are then combined for 3-D analyses. For biological specimens, tomography enables the highest 3-D resolution (5 nm spatial resolution) of internal structures in relatively thick slices of material (0.2-0.4 μm) without requiring the collection and alignment of large numbers of thin serial sections. Thus accurate and revealing 3-D reconstructions of complex cytoplasmic entities and architecture can be obtained. Electron tomography is now being applied to a variety of biological questions with great success. This review gives a brief introduction into cryopreservation and electron tomography relative to aspects of cytoplasmic organization in the hyphal tip of Aspergillus nidulans.

Original languageEnglish (US)
Pages (from-to)208-220
Number of pages13
JournalNew Phytologist
Volume172
Issue number2
DOIs
StatePublished - Oct 2006

Fingerprint

Electron Microscope Tomography
tomography
Tomography
ultrastructure
electrons
transmission electron microscopes
Electrons
Electron microscopes
Biological systems
Aspergillus nidulans
Access to Information
Biological Science Disciplines
Cryopreservation
Specimen preparation
Computerized tomography
Aspergillus
Organizations
instrumentation
cryopreservation
image analysis

Keywords

  • Aspergillus nidulans
  • Cryofixation
  • Cytoskeleton
  • Electron tomography
  • Spitzenkörper

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Hohmann-Marriott, M. F., Uchida, M., Van De Meene, A. M. L., Garret, M., Hjelm, B. E., Kokoori, S., & Roberson, R. (2006). Application of electron tomography to fungal ultrastructure studies. New Phytologist, 172(2), 208-220. https://doi.org/10.1111/j.1469-8137.2006.01868.x

Application of electron tomography to fungal ultrastructure studies. / Hohmann-Marriott, Martin F.; Uchida, Maho; Van De Meene, Allison M L; Garret, Matthew; Hjelm, Brooke E.; Kokoori, Shylaja; Roberson, Robert.

In: New Phytologist, Vol. 172, No. 2, 10.2006, p. 208-220.

Research output: Contribution to journalArticle

Hohmann-Marriott, MF, Uchida, M, Van De Meene, AML, Garret, M, Hjelm, BE, Kokoori, S & Roberson, R 2006, 'Application of electron tomography to fungal ultrastructure studies', New Phytologist, vol. 172, no. 2, pp. 208-220. https://doi.org/10.1111/j.1469-8137.2006.01868.x
Hohmann-Marriott MF, Uchida M, Van De Meene AML, Garret M, Hjelm BE, Kokoori S et al. Application of electron tomography to fungal ultrastructure studies. New Phytologist. 2006 Oct;172(2):208-220. https://doi.org/10.1111/j.1469-8137.2006.01868.x
Hohmann-Marriott, Martin F. ; Uchida, Maho ; Van De Meene, Allison M L ; Garret, Matthew ; Hjelm, Brooke E. ; Kokoori, Shylaja ; Roberson, Robert. / Application of electron tomography to fungal ultrastructure studies. In: New Phytologist. 2006 ; Vol. 172, No. 2. pp. 208-220.
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