Application of displacement thin-layer chromatography to toad-poison bufadienolides

Yoshiaki Kamano, Ayano Kotake, Toshihiko Nogawa, Machiko Tozawa, George Pettit

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have investigated displacement thin-layer chromatography with organic bases N,N-dimethyl-1,3-propanediamine, N,N-dimethylcyclohexylamine, and triethylamine for the separation of seven toad-poison bufadienolides - resibufogenin, cinobufagin, bufalin, bufotalin, cinobufotalin, telocinobufagin, and gamabufotalin. The multiple use of the mobile phase mixtures n-hexane-acetone, n-hexane-ethyl acetate, n-hexane-dichloromethane- acetone, n-hexane-dichloromethane-methanol, and n-hexane-ethyl acetate- methanol, each containing 3% (v/v) organic bases, furnished effective separation of the bufadienolides. The characteristics of the separations and the visualization of the bufadienolides by derivatization are also discussed. The technique has been applied to the analysis of the major bufadienolides in the Chinese traditional drug Ch'an Su.

Original languageEnglish (US)
Pages (from-to)120-123
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume12
Issue number2
StatePublished - Mar 1999

Fingerprint

Bufanolides
Thin layer chromatography
Poisons
Thin Layer Chromatography
Anura
Methylene Chloride
Acetone
Methanol
Visualization
n-hexane
Pharmaceutical Preparations

Keywords

  • Bufadienolides
  • Ch'an Su
  • Displacement TLC
  • Multiple one-dimensional separation
  • Multiple two- dimensional separation

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Application of displacement thin-layer chromatography to toad-poison bufadienolides. / Kamano, Yoshiaki; Kotake, Ayano; Nogawa, Toshihiko; Tozawa, Machiko; Pettit, George.

In: Journal of Planar Chromatography - Modern TLC, Vol. 12, No. 2, 03.1999, p. 120-123.

Research output: Contribution to journalArticle

Kamano, Yoshiaki ; Kotake, Ayano ; Nogawa, Toshihiko ; Tozawa, Machiko ; Pettit, George. / Application of displacement thin-layer chromatography to toad-poison bufadienolides. In: Journal of Planar Chromatography - Modern TLC. 1999 ; Vol. 12, No. 2. pp. 120-123.
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