Applicability of quantitative PCR for determination of removal efficacy of enteric viruses and Cryptosporidium by water treatment processes

Hodon Ryu, Brooke Mayer, Morteza Abbaszadegan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.

Original languageEnglish (US)
Pages (from-to)101-108
Number of pages8
JournalJournal of Water and Health
Volume8
Issue number1
DOIs
StatePublished - 2010

Fingerprint

Cryptosporidium
Enterovirus
Water Purification
water treatment
virus
Polymerase Chain Reaction
detection method
Human Enterovirus B
coagulation
microorganism
Adenoviridae
assay
Fluorescent Antibody Technique
Cell Culture Techniques
Viruses
removal
method

Keywords

  • Cryptosporidium
  • Enteric viruses
  • Quantitative PCR
  • Water treatment processes

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Infectious Diseases
  • Microbiology (medical)
  • Public Health, Environmental and Occupational Health

Cite this

@article{3d838d21996c4854ba72eca28d55c030,
title = "Applicability of quantitative PCR for determination of removal efficacy of enteric viruses and Cryptosporidium by water treatment processes",
abstract = "This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.",
keywords = "Cryptosporidium, Enteric viruses, Quantitative PCR, Water treatment processes",
author = "Hodon Ryu and Brooke Mayer and Morteza Abbaszadegan",
year = "2010",
doi = "10.2166/wh.2009.208",
language = "English (US)",
volume = "8",
pages = "101--108",
journal = "Journal of Water and Health",
issn = "1477-8920",
publisher = "IWA Publishing",
number = "1",

}

TY - JOUR

T1 - Applicability of quantitative PCR for determination of removal efficacy of enteric viruses and Cryptosporidium by water treatment processes

AU - Ryu, Hodon

AU - Mayer, Brooke

AU - Abbaszadegan, Morteza

PY - 2010

Y1 - 2010

N2 - This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.

AB - This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.

KW - Cryptosporidium

KW - Enteric viruses

KW - Quantitative PCR

KW - Water treatment processes

UR - http://www.scopus.com/inward/record.url?scp=77949368977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949368977&partnerID=8YFLogxK

U2 - 10.2166/wh.2009.208

DO - 10.2166/wh.2009.208

M3 - Article

VL - 8

SP - 101

EP - 108

JO - Journal of Water and Health

JF - Journal of Water and Health

SN - 1477-8920

IS - 1

ER -