Abstract
This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 101-108 |
| Number of pages | 8 |
| Journal | Journal of Water and Health |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2010 |
Keywords
- Cryptosporidium
- Enteric viruses
- Quantitative PCR
- Water treatment processes
ASJC Scopus subject areas
- Water Science and Technology
- Waste Management and Disposal
- Public Health, Environmental and Occupational Health
- Microbiology (medical)
- Infectious Diseases