Applicability of quantitative PCR for determination of removal efficacy of enteric viruses and Cryptosporidium by water treatment processes

Hodon Ryu, Brooke Mayer, Morteza Abbaszadegan

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.

Original languageEnglish (US)
Pages (from-to)101-108
Number of pages8
JournalJournal of Water and Health
Volume8
Issue number1
DOIs
StatePublished - Mar 19 2010

Keywords

  • Cryptosporidium
  • Enteric viruses
  • Quantitative PCR
  • Water treatment processes

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

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