Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level

Nilsa A. Bosque-Pérez, Gregory D. May, Charles J. Arntzen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains.

Original languageEnglish (US)
Title of host publicationActa Horticulturae
Pages193-201
Number of pages9
Volume540
StatePublished - 2000
Externally publishedYes

Publication series

NameActa Horticulturae
Volume540
ISSN (Print)05677572

Fingerprint

Musa
ploidy
Agrobacterium
bananas
genomics
plantains (fruit)
highlands
diploidy
Musa balbisiana
clones
desserts
gene transfer
reporter genes
cooking
cultivars
assays
genes

Keywords

  • Agrobacterium tumefaciens
  • Cooking bananas
  • Dessert bananas
  • Plant transformation
  • Plantains

ASJC Scopus subject areas

  • Horticulture

Cite this

Bosque-Pérez, N. A., May, G. D., & Arntzen, C. J. (2000). Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level. In Acta Horticulturae (Vol. 540, pp. 193-201). (Acta Horticulturae; Vol. 540).

Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level. / Bosque-Pérez, Nilsa A.; May, Gregory D.; Arntzen, Charles J.

Acta Horticulturae. Vol. 540 2000. p. 193-201 (Acta Horticulturae; Vol. 540).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bosque-Pérez, NA, May, GD & Arntzen, CJ 2000, Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level. in Acta Horticulturae. vol. 540, Acta Horticulturae, vol. 540, pp. 193-201.
Bosque-Pérez NA, May GD, Arntzen CJ. Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level. In Acta Horticulturae. Vol. 540. 2000. p. 193-201. (Acta Horticulturae).
Bosque-Pérez, Nilsa A. ; May, Gregory D. ; Arntzen, Charles J. / Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level. Acta Horticulturae. Vol. 540 2000. pp. 193-201 (Acta Horticulturae).
@inproceedings{846e83afbb7649e5998e7804d530e9dd,
title = "Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level",
abstract = "An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains.",
keywords = "Agrobacterium tumefaciens, Cooking bananas, Dessert bananas, Plant transformation, Plantains",
author = "Bosque-P{\'e}rez, {Nilsa A.} and May, {Gregory D.} and Arntzen, {Charles J.}",
year = "2000",
language = "English (US)",
isbn = "9789066059535",
volume = "540",
series = "Acta Horticulturae",
pages = "193--201",
booktitle = "Acta Horticulturae",

}

TY - GEN

T1 - Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level

AU - Bosque-Pérez, Nilsa A.

AU - May, Gregory D.

AU - Arntzen, Charles J.

PY - 2000

Y1 - 2000

N2 - An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains.

AB - An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains.

KW - Agrobacterium tumefaciens

KW - Cooking bananas

KW - Dessert bananas

KW - Plant transformation

KW - Plantains

UR - http://www.scopus.com/inward/record.url?scp=51649100951&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51649100951&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:51649100951

SN - 9789066059535

VL - 540

T3 - Acta Horticulturae

SP - 193

EP - 201

BT - Acta Horticulturae

ER -