Abstract

Viral infections elicit antiviral antibodies and have been associated with various chronic diseases. Detection of these antibodies can facilitate diagnosis, treatment of infection, and understanding of the mechanisms of virus-associated diseases. In this work, we assayed antiviral antibodies using a novel high-density nucleic acid programmable protein array (HD-NAPPA) platform. Individual viral proteins were expressed in situ directly from plasmids encoding proteins in an array of microscopic reaction chambers. Quality of protein display and serum response was assured by comparing intra- and inter-array correlation within or between printing batches with average correlation coefficients of 0.91 and 0.96, respectively. HD-NAPPA showed higher signal-to-background ratio compared with standard NAPPA on planar glass slides and ELISA. Antibody responses to 761 antigens from 25 different viruses were profiled among patients with juvenile idiopathic arthritis and type 1 diabetes. Common and unique antibody reactivity patterns were detected between patients and healthy controls. We believe HD-viral-NAPPA will enable the study of host-pathogen interactions at unprecedented dimensions and elucidate the role of pathogen infections in disease development.

Original languageEnglish (US)
Pages (from-to)2136-2145
Number of pages10
JournalProteomics
Volume15
Issue number12
DOIs
StatePublished - Jun 1 2015

Fingerprint

Protein Array Analysis
Antiviral Agents
Antibodies
Virus Diseases
Nucleic Acids
Pathogens
Proteins
Viruses
Host-Pathogen Interactions
Printing
Juvenile Arthritis
Viral Proteins
Infection
Type 1 Diabetes Mellitus
Antibody Formation
Glass
Blood Proteins
Medical problems
Plasmids
Chronic Disease

Keywords

  • Antiviral antibodies
  • HD-NAPPA
  • Juvenile idiopathic arthritis
  • Protein arrays
  • Type 1 diabetes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Bian, X., Wiktor, P., Kahn, P., Brunner, A., Khela, A., Karthikeyan, K., ... LaBaer, J. (2015). Antiviral antibody profiling by high-density protein arrays. Proteomics, 15(12), 2136-2145. https://doi.org/10.1002/pmic.201400612

Antiviral antibody profiling by high-density protein arrays. / Bian, Xiaofang; Wiktor, Peter; Kahn, Peter; Brunner, Al; Khela, Amritpal; Karthikeyan, Kailash; Barker, Kristi; Yu, Xiaobo; Magee, Dewey; Wasserfall, Clive H.; Gibson, David; Rooney, Madeleine E.; Qiu, Ji; LaBaer, Joshua.

In: Proteomics, Vol. 15, No. 12, 01.06.2015, p. 2136-2145.

Research output: Contribution to journalArticle

Bian, X, Wiktor, P, Kahn, P, Brunner, A, Khela, A, Karthikeyan, K, Barker, K, Yu, X, Magee, D, Wasserfall, CH, Gibson, D, Rooney, ME, Qiu, J & LaBaer, J 2015, 'Antiviral antibody profiling by high-density protein arrays', Proteomics, vol. 15, no. 12, pp. 2136-2145. https://doi.org/10.1002/pmic.201400612
Bian X, Wiktor P, Kahn P, Brunner A, Khela A, Karthikeyan K et al. Antiviral antibody profiling by high-density protein arrays. Proteomics. 2015 Jun 1;15(12):2136-2145. https://doi.org/10.1002/pmic.201400612
Bian, Xiaofang ; Wiktor, Peter ; Kahn, Peter ; Brunner, Al ; Khela, Amritpal ; Karthikeyan, Kailash ; Barker, Kristi ; Yu, Xiaobo ; Magee, Dewey ; Wasserfall, Clive H. ; Gibson, David ; Rooney, Madeleine E. ; Qiu, Ji ; LaBaer, Joshua. / Antiviral antibody profiling by high-density protein arrays. In: Proteomics. 2015 ; Vol. 15, No. 12. pp. 2136-2145.
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