Antisomatostatin gamma globulin augments secretion of both insulin and glucagon in vitro. Evidence for a physiologic role for endogenous somatostatin in the regulation of pancreatic A- and B-cell function

M. Itoh, L. Mandarino, J. E. Gerich

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    Abstract

    To assess the effects of endogenous somatostatin on pancreatic islet A- and B-cell function, isolated rat islets were incubated in antisomatostatin γ-globulin to bind endogenously released somatostatin, and the insulin and glucagon secretion of these islets was compared with that of islets incubated in γ-globulin isolated from nonimmune serum. Islets incubated in antisomatostatin γ-globulin released significantly more insulin at 4, 8, 16, and 32 mM glucose and significantly more glucagon at 8, 16, and 32 mM glucose, P < 0.05-0.005. For glucose-stimulated insulin release the threshold was decreased, the V(max) was increased, but the apparent K(m) was unaltered; for glucose-suppression of glucagon release the threshold was increased, maximal suppression was decreased, but the apparent K(i) was unaltered. The augmentative effect of the antisomatostatin γ-globulin was most prominent at 4 mM glucose for insulin release and at 8 mM glucose for glucagon release, but was not limited to glucose since both insulin and glucagon release stimulated by arginine were also augmented by antisomatostatin γ-globulin. These results provide evidence that endogenous somatostatin may act as a physiologic local regulator of both insulin and glucagon secretion and that its effect on insulin and glucagon secretion is dependent on the prevailing glucose concentration.

    Original languageEnglish (US)
    Pages (from-to)693-696
    Number of pages4
    JournalDiabetes
    Volume29
    Issue number9
    DOIs
    StatePublished - Jan 1 1980

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    ASJC Scopus subject areas

    • Internal Medicine
    • Endocrinology, Diabetes and Metabolism

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