Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes

Rui Huo, Yi Shi, Jun Jie Xu, Fei Yan, Shao Wu Lü, Jia Ming Su, Yu Jing Duan, Jia Fan, Bo Ning, Deng Li Cong, Gang Lin Yan, Gui Min Luo, Jing Yan Wei

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Reactive oxygen species(ROS) plays a key role in human heart diseases. Glutathione peroxidase(GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide(H2O2)-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H2O2 level. The Se-scFv-B3 significantly reduced H2O2-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde(MDA) production, lactate dehydrogenase(LDH) release, and intracellular H2O2 level. So Se-scFv- B3 may have a great potential in the treatment of human heart diseases induced by ROS.

Original languageEnglish (US)
Pages (from-to)216-219
Number of pages4
JournalChemical Research in Chinese Universities
Volume25
Issue number2
StatePublished - Dec 1 2009

Keywords

  • Cardiac myocyte
  • Glutathione peroxidase
  • HO
  • Human single-chain Fv
  • Selenium

ASJC Scopus subject areas

  • Chemistry(all)

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    Huo, R., Shi, Y., Xu, J. J., Yan, F., Lü, S. W., Su, J. M., Duan, Y. J., Fan, J., Ning, B., Cong, D. L., Yan, G. L., Luo, G. M., & Wei, J. Y. (2009). Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes. Chemical Research in Chinese Universities, 25(2), 216-219.