Abstract
Reactive oxygen species(ROS) plays a key role in human heart diseases. Glutathione peroxidase(GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide(H2O2)-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H2O2 level. The Se-scFv-B3 significantly reduced H2O2-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde(MDA) production, lactate dehydrogenase(LDH) release, and intracellular H2O2 level. So Se-scFv- B3 may have a great potential in the treatment of human heart diseases induced by ROS.
Original language | English (US) |
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Pages (from-to) | 216-219 |
Number of pages | 4 |
Journal | Chemical Research in Chinese Universities |
Volume | 25 |
Issue number | 2 |
State | Published - 2009 |
Externally published | Yes |
Keywords
- Cardiac myocyte
- Glutathione peroxidase
- HO
- Human single-chain Fv
- Selenium
ASJC Scopus subject areas
- General Chemistry