Antioxidant cerium oxide nanoparticle hydrogels for cellular encapsulation

Jessica D. Weaver, Cherie L. Stabler

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation. Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported. This enzyme-mimetic material ubiquitously scavenges ambient free radicals, with the potential to provide indefinite antioxidant protection. The potential of this system to enhance the protection of encapsulated beta cells was evaluated. Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1 mM. When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10 mM. This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging.

Original languageEnglish (US)
Pages (from-to)136-144
Number of pages9
JournalActa Biomaterialia
Volume16
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Hydrogels
Hydrogel
Cerium
Antioxidants
Encapsulation
Nanoparticles
Cytotoxicity
Free Radicals
Oxides
Cytoprotection
Free radicals
Transplantation (surgical)
Oxidative stress
Transplantation
Scavenging
Composite materials
Nanocomposites
Grafts
Superoxides
Byproducts

Keywords

  • Alginate
  • Beta cell
  • Free radical
  • Immunoisolation
  • Nanoceria

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Antioxidant cerium oxide nanoparticle hydrogels for cellular encapsulation. / Weaver, Jessica D.; Stabler, Cherie L.

In: Acta Biomaterialia, Vol. 16, No. 1, 01.01.2015, p. 136-144.

Research output: Contribution to journalArticle

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