Antineoplastic agents 596. Isolation and structure of chromomycin A5 from a Beaufort Sea microorganism

George Pettit, Rui Tan, Robin Pettit, Dennis L. Doubek, Jean Chapuis, Christine A. Weber

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A microorganism identified as Streptomyces sp. was isolated from a sedimentary specimen collected on the Beaufort Sea coast of Alaska. Fermentation scale-up and cancer cell line-guided separation of the constituents led to isolation and structural elucidation (high resolution MALDI-TOF mass and 2D NMR spectral analyses) of a diastereomer of chromomycin A2 (2) designated chromomycin A5 (5). Chromomycin A5 displayed very potent cancer cell growth inhibitory activity (reaching with MCF-7 breast cancer GI50 0.00073 μg mL-1) against a mini-panel of murine and human cancer cell lines.

Original languageEnglish (US)
Pages (from-to)9116-9122
Number of pages7
JournalRSC Advances
Volume5
Issue number12
DOIs
StatePublished - 2015

Fingerprint

Chromomycins
Microorganisms
Antineoplastic Agents
Cells
Cell growth
Fermentation
Coastal zones
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Antineoplastic agents 596. Isolation and structure of chromomycin A5 from a Beaufort Sea microorganism. / Pettit, George; Tan, Rui; Pettit, Robin; Doubek, Dennis L.; Chapuis, Jean; Weber, Christine A.

In: RSC Advances, Vol. 5, No. 12, 2015, p. 9116-9122.

Research output: Contribution to journalArticle

@article{3c5cf084c89d4ae884cb5790b842ad44,
title = "Antineoplastic agents 596. Isolation and structure of chromomycin A5 from a Beaufort Sea microorganism",
abstract = "A microorganism identified as Streptomyces sp. was isolated from a sedimentary specimen collected on the Beaufort Sea coast of Alaska. Fermentation scale-up and cancer cell line-guided separation of the constituents led to isolation and structural elucidation (high resolution MALDI-TOF mass and 2D NMR spectral analyses) of a diastereomer of chromomycin A2 (2) designated chromomycin A5 (5). Chromomycin A5 displayed very potent cancer cell growth inhibitory activity (reaching with MCF-7 breast cancer GI50 0.00073 μg mL-1) against a mini-panel of murine and human cancer cell lines.",
author = "George Pettit and Rui Tan and Robin Pettit and Doubek, {Dennis L.} and Jean Chapuis and Weber, {Christine A.}",
year = "2015",
doi = "10.1039/c4ra16517a",
language = "English (US)",
volume = "5",
pages = "9116--9122",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "12",

}

TY - JOUR

T1 - Antineoplastic agents 596. Isolation and structure of chromomycin A5 from a Beaufort Sea microorganism

AU - Pettit, George

AU - Tan, Rui

AU - Pettit, Robin

AU - Doubek, Dennis L.

AU - Chapuis, Jean

AU - Weber, Christine A.

PY - 2015

Y1 - 2015

N2 - A microorganism identified as Streptomyces sp. was isolated from a sedimentary specimen collected on the Beaufort Sea coast of Alaska. Fermentation scale-up and cancer cell line-guided separation of the constituents led to isolation and structural elucidation (high resolution MALDI-TOF mass and 2D NMR spectral analyses) of a diastereomer of chromomycin A2 (2) designated chromomycin A5 (5). Chromomycin A5 displayed very potent cancer cell growth inhibitory activity (reaching with MCF-7 breast cancer GI50 0.00073 μg mL-1) against a mini-panel of murine and human cancer cell lines.

AB - A microorganism identified as Streptomyces sp. was isolated from a sedimentary specimen collected on the Beaufort Sea coast of Alaska. Fermentation scale-up and cancer cell line-guided separation of the constituents led to isolation and structural elucidation (high resolution MALDI-TOF mass and 2D NMR spectral analyses) of a diastereomer of chromomycin A2 (2) designated chromomycin A5 (5). Chromomycin A5 displayed very potent cancer cell growth inhibitory activity (reaching with MCF-7 breast cancer GI50 0.00073 μg mL-1) against a mini-panel of murine and human cancer cell lines.

UR - http://www.scopus.com/inward/record.url?scp=84921300548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921300548&partnerID=8YFLogxK

U2 - 10.1039/c4ra16517a

DO - 10.1039/c4ra16517a

M3 - Article

AN - SCOPUS:84921300548

VL - 5

SP - 9116

EP - 9122

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 12

ER -