Antineoplastic Agents. 499. Synthesis of Hystatin 2 and Related 1H-Benzo[de][1,6]-naphthyridinium Salts from Aaptamine

George Pettit, Holger Hoffmann, Delbert L. Herald, Peter M. Blumberg, Ernest Hamel, Jean M. Schmidt, Yung Chang, Robin Pettit, Nancy E. Lewin, Larry V. Pearce

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The marine sponge constituent aaptamine (1) has been converted to the cancer cell growth inhibitor and antibiotic designated hystatin 2 (8a). Herein, we also report results of an initial SAR evaluation of new benzyl derivatives of aaptamine (1). Single benzylation was found to occur at nitrogen N-4 and led to the formation of the 4-benzylaaptamine derivatives 7a-c, whereas double benzylation gave the quaternary 1H-benzo[de][1,6]-naphthyridinium salts 8a-c. The anticancer and antimicrobial properties of these aaptamine derivatives are described. The quaternary ammonium salts 8a (hystatin 2) and 8b exhibited significant inhibitory activity against the murine P388 lymphocytic leukemia and a minipanel of human cancer cell lines. Salts 8a and 8b also had broad spectrum antimicrobial activities and were most potent against Mycobacterium tuberculosis, Neisseria gonorrhoeae, and Micrococcus luteus. Naphthyridinium chloride 8a was selected for further development, and results of an initial cell cycle analysis and a cDNA microarray study showed effects consistent with inhibition of the S-phase of cell growth.

Original languageEnglish (US)
Pages (from-to)1775-1782
Number of pages8
JournalJournal of Medicinal Chemistry
Volume47
Issue number7
DOIs
StatePublished - Mar 25 2004

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

Fingerprint Dive into the research topics of 'Antineoplastic Agents. 499. Synthesis of Hystatin 2 and Related 1H-Benzo[de][1,6]-naphthyridinium Salts from Aaptamine'. Together they form a unique fingerprint.

  • Cite this