Antigenic diversity of the Plasmodium vivax circumsporozoite protein in parasite isolates of Western Colombia

Miguel Ángel Hernández-Martínez, Ananiás A. Escalante, Myriam Arev́alo-Herrera, Soćrates Herrera

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Circumsporozoite (CS) protein is a malaria antigen involved in sporozoite invasion of hepatocytes, and thus considered to have good vaccine potential. We evaluated the polymorphism of the Plasmodium vivax CS gene in 24 parasite isolates collected from malaria-endemic areas of Colombia. We sequenced 27 alleles, most of which (25/27) corresponded to the VK247 genotype and the remainder to the VK210 type. All VK247 alleles presented a mutation (Gly → Asn) at position 28 in the N-terminal region, whereas the C-terminal presented three insertions: the ANKKAGDAG, which is common in all VK247 isolates; 12 alleles presented the insertion GAGGQAAGGNAANKKAGDAG; and 5 alleles presented the insertion GGNAGGNA. Both repeat regions were polymorphic in gene sequence and size. Sequences coding for B-, T-CD4 +, and T-CD8 + cell epitopes were found to be conserved. This study confirms the high polymorphism of the repeat domain and the highly conserved nature of the flanking regions.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume84
Issue number2 S
DOIs
StatePublished - Feb 1 2011

    Fingerprint

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Cite this