Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Shaopeng Wang, Natalia Mamedova, Nicholas A. Kotov, Wei Chen, Joe Studer

Research output: Contribution to journalArticle

517 Scopus citations

Abstract

Complementary bioconjugates based on antibody-antigen interactions were synthesized from luminescent CdTe nanoparticles (NPs). Antigen (bovine serum albumin) was conjugated to red-emitting CdTe NPs, while green-emitting NPs were attached to the corresponding anti-BSA antibody (IgG). The NP bioconjugates were characterized by native and SDS-PAGE electrophoresis, gel-permeation HPLC, and circular dichroism. Antigen-antibody binding affinity was evaluated by enzyme-linked immunosorbent assay (ELISA). The formation of BSA-IgG immunocomplex resulted in the Förster resonance energy transfer (FRET) between the two different NPs: the luminescence of green-emitting NPs was quenched whereas the emission of the red-emitting NPs was enhanced. The luminescence recovered when the immunocomplex was exposed to an unlabeled antigen. The immunocomplexes can be considered as a prototype of NP superstructures based on biospecific ligands, while the competitive FRET inhibition can be used in an immunoassay protocol.

Original languageEnglish (US)
Pages (from-to)817-822
Number of pages6
JournalNano Letters
Volume2
Issue number8
DOIs
StatePublished - Aug 1 2002

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ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Wang, S., Mamedova, N., Kotov, N. A., Chen, W., & Studer, J. (2002). Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates. Nano Letters, 2(8), 817-822. https://doi.org/10.1021/nl0255193