Antigen-specific lymphoproliferative responses to tetanus toxoid

A ans for the evaluation of Marek's disease virus-induced immunosuppression in chickens

S. K. Reddy, M. Suresh, K. Karaca, J. M. Sharma, J. McMillen, R. D. Schwartz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Antigen-specific lymphoproliferative responses were examined in chickens following immunization with tetanus toxoid (Ttx). The immune competence of chickens was assessed by mitogen assay utilizing phytohemagglutinin (PHA)-stimulation and Ttx-specific antigen proliferation assay (Ttx-APA). Immune spleen cells but not peripheral blood leucocytes demonstrated specific proliferation following stimulation in vitro in a Ttx-APA. In this study, we examined firstly the effects of Marek's disease (MD) associated immunosuppression on specific immune responses. The humoral and cell-mediated immune responses were monitored by enzyme-linked immunosorbent assay (ELISA) and Ttx-APA, respectively. Secondly, we examined if vaccination against MD using a conventional herpesvirus of turkeys (HVT) vaccine and two recombinant HVT (rHVT) vaccines would affect the development of Ttx-specific immune responses. The rHVT vaccines used in this study included two constructs: one expressing both Newcastle disease virus (NDV) and MD virus (MDV) genes (HVT/NDV/MDV), and another expressing only MDV genes (HVT/MDV). The mitogenic responses of spleen cells of the vaccinated chickens were inconsistent allowing no definitive conclusions about vaccinal immunosuppression. The results of the Ttx-APA indicated that Ttx-specific lymphoproliferative responses provide a meaningful measure of immunosuppression. The MDV-induced immunosuppression resulted in the inhibition of Ttx-specific lymphoproliferation in vitro. Both HVT and rHVT vaccines were not immunosuppressive as indicated by the development of normal Ttx-specific lymphoproliferative responses in chickens. These results indicate that vaccination against MD results not only in the prevention of tumor formation but also protection from possible virus-induced immunosuppression.

Original languageEnglish (US)
Pages (from-to)1695-1702
Number of pages8
JournalVaccine
Volume14
Issue number17-18
DOIs
StatePublished - Dec 1996
Externally publishedYes

Fingerprint

Marek Disease
Mardivirus
Tetanus Toxoid
toxoids
tetanus
immunosuppression
Immunosuppression
Chickens
Meleagrid herpesvirus 1
chickens
Viruses
antigens
Antigens
Meleagrid Herpesvirus 1
Synthetic Vaccines
Marek disease
Herpesvirus Vaccines
viruses
Newcastle disease virus
recombinant vaccines

Keywords

  • antigenic proliferation assay
  • fusion and hemagglutinin-neuraminidase of Newcastle disease virus
  • glycoprotein A, B and D of Marek's disease virus
  • PHA-P
  • recombinant herpesvirus of turkeys
  • tetanus toxoid

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Antigen-specific lymphoproliferative responses to tetanus toxoid : A ans for the evaluation of Marek's disease virus-induced immunosuppression in chickens. / Reddy, S. K.; Suresh, M.; Karaca, K.; Sharma, J. M.; McMillen, J.; Schwartz, R. D.

In: Vaccine, Vol. 14, No. 17-18, 12.1996, p. 1695-1702.

Research output: Contribution to journalArticle

Reddy, S. K. ; Suresh, M. ; Karaca, K. ; Sharma, J. M. ; McMillen, J. ; Schwartz, R. D. / Antigen-specific lymphoproliferative responses to tetanus toxoid : A ans for the evaluation of Marek's disease virus-induced immunosuppression in chickens. In: Vaccine. 1996 ; Vol. 14, No. 17-18. pp. 1695-1702.
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