TY - JOUR
T1 - Antibacterial activity and membrane-disrupting mechanism of monocaprin against Escherichia coli and its application in apple and carrot juices
AU - Ma, Maomao
AU - Zhao, Junxin
AU - Zeng, Zheling
AU - Wan, Dongman
AU - Yu, Ping
AU - Cheng, Ding
AU - Gong, Deming
AU - Deng, Shuguang
N1 - Funding Information:
This work was supported by the International Science and Technology Cooperation Programme of China ( 2011DFA32770 ), the Research Program of State Key Laboratory of Food Science and Technology , Nanchang University ( SKLF-ZZB-201517 , SKLF-ZZA-201610 , SKLF-ZZB-201916 , SKLF-KF-201812 ), the Science and Technology Program of Jiangxi Province ( 20143ACG70015 ), the National Natural Science Foundation of China ( 31701651 ).
Publisher Copyright:
© 2020
PY - 2020/9
Y1 - 2020/9
N2 - In this study, the antibacterial activity of monocaprin against Gram-negative foodborne pathogens and its underlying mechanism against E. coli were investigated. It was found that monocaprin exerted excellent antibacterial effects on E. coli, Vibrio parahaemolyticus and Shigella dysenteriae with the minimum inhibitory concentration/minimum bactericidal concentration values of 1.25 mM, 0.625 mM and 0.625 mM, respectively. The results of growth curve and time-kill assay showed that monocaprin could not only delay the growth of E. coli, but quickly inactivate the cells within 15 min. The effects of monocaprin on membrane integrity, inner and outer membrane permeability, proton motive force, and its interaction with model membranes and cell morphology were investigated to explore the antibacterial mechanism of monocaprin. The results indicated that monocaprin rapidly inactivated E. coli by penetrating its outer membrane, then destroying the phospholipid bilayer structure of the inner membrane, as well as dissipating the proton motive force, resulting in increases of the permeability of the outer and inner membranes and leakage of intracellular substances. Besides, monocaprin significantly reduced the viability of E. coli in apple and carrot juices at 4 °C and 25 °C without changing their sensory properties.
AB - In this study, the antibacterial activity of monocaprin against Gram-negative foodborne pathogens and its underlying mechanism against E. coli were investigated. It was found that monocaprin exerted excellent antibacterial effects on E. coli, Vibrio parahaemolyticus and Shigella dysenteriae with the minimum inhibitory concentration/minimum bactericidal concentration values of 1.25 mM, 0.625 mM and 0.625 mM, respectively. The results of growth curve and time-kill assay showed that monocaprin could not only delay the growth of E. coli, but quickly inactivate the cells within 15 min. The effects of monocaprin on membrane integrity, inner and outer membrane permeability, proton motive force, and its interaction with model membranes and cell morphology were investigated to explore the antibacterial mechanism of monocaprin. The results indicated that monocaprin rapidly inactivated E. coli by penetrating its outer membrane, then destroying the phospholipid bilayer structure of the inner membrane, as well as dissipating the proton motive force, resulting in increases of the permeability of the outer and inner membranes and leakage of intracellular substances. Besides, monocaprin significantly reduced the viability of E. coli in apple and carrot juices at 4 °C and 25 °C without changing their sensory properties.
KW - Antibacterial activity
KW - Application
KW - Escherichia coli
KW - Mechanism
KW - Monocaprin
UR - http://www.scopus.com/inward/record.url?scp=85087201618&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087201618&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2020.109794
DO - 10.1016/j.lwt.2020.109794
M3 - Article
AN - SCOPUS:85087201618
SN - 0023-6438
VL - 131
JO - LWT
JF - LWT
M1 - 109794
ER -