Antibacterial activity and action mechanism of microencapsulated dodecyl gallate with methyl-β-cyclodextrin

Junxin Zhao, Ting Peng, Shibo Liang, Maomao Ma, Zheling Zeng, Ping Yu, Deming Gong, Shuguang Deng

Research output: Contribution to journalArticle

Abstract

The application of dodecyl gallate (DG) is limited due to its poor stability and water-solubility. A new compound of DG microencapsulated with methyl-β-cyclodextrin (MβCD), MβCD-DG was produced, and the inhibition effects of MβCD-DG against three common foodborne pathogens (Staphylococcus aureus, Bacillus cereus and Bacillus subtilis) were investigated in the study. The antioxidant activity of MβCD-DG was determined by the DPPH method (EC50 = 4.515 ± 0.5 μg/mL). The minimum inhibitory concentration (MIC) of MβCD-DG against these bacteria were 0.11, 0.06 and 0.06 mg/mL, and minimum bactericidal concentration (MBC) were 0.22, 0.11 and 0.11 mg/mL, respectively. The MICs remained steady under different pH values (3.0–9.0) and temperatures (4 and 25 °C), indicating that the anti-bacterial effect of MβCD-DG was independent of pH and temperature. Measurements of permeability of cell wall and membrane, membrane potential, release of cellular contents and observation of cell morphology were carried out to explore the antibacterial mechanism of MβCD-DG. The results indicated that MβCD-DG inhibited S. aureus by increasing the permeability of cell membrane and leakage of cellular contents without obvious cell morphological changes. MβCD-DG may kill B. cereus and B. subtilis by damaging the cell wall and cytoplasmic membrane to make the release of cellular contents. As a novel preservative, MβCD-DG is more efficient than DG and common chemical additives, and is thus worthy of further exploitation in food industry.

Original languageEnglish (US)
Article number106953
JournalFood Control
Volume109
DOIs
StatePublished - Mar 2020

Fingerprint

cyclodextrins
Cyclodextrins
mechanism of action
Bacillus cereus
Bacillus subtilis
Cell Membrane
cell membranes
Staphylococcus aureus
Cell Wall
cell walls
lauryl gallate
Cell Membrane Permeability
water solubility
membrane permeability
food pathogens
minimum inhibitory concentration
preservatives
Temperature
membrane potential
Food Industry

Keywords

  • Antibacterial mechanism
  • Dodecyl gallate
  • Foodborne pathogens
  • Microcapsule
  • Preservative

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Antibacterial activity and action mechanism of microencapsulated dodecyl gallate with methyl-β-cyclodextrin. / Zhao, Junxin; Peng, Ting; Liang, Shibo; Ma, Maomao; Zeng, Zheling; Yu, Ping; Gong, Deming; Deng, Shuguang.

In: Food Control, Vol. 109, 106953, 03.2020.

Research output: Contribution to journalArticle

Zhao, Junxin ; Peng, Ting ; Liang, Shibo ; Ma, Maomao ; Zeng, Zheling ; Yu, Ping ; Gong, Deming ; Deng, Shuguang. / Antibacterial activity and action mechanism of microencapsulated dodecyl gallate with methyl-β-cyclodextrin. In: Food Control. 2020 ; Vol. 109.
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abstract = "The application of dodecyl gallate (DG) is limited due to its poor stability and water-solubility. A new compound of DG microencapsulated with methyl-β-cyclodextrin (MβCD), MβCD-DG was produced, and the inhibition effects of MβCD-DG against three common foodborne pathogens (Staphylococcus aureus, Bacillus cereus and Bacillus subtilis) were investigated in the study. The antioxidant activity of MβCD-DG was determined by the DPPH method (EC50 = 4.515 ± 0.5 μg/mL). The minimum inhibitory concentration (MIC) of MβCD-DG against these bacteria were 0.11, 0.06 and 0.06 mg/mL, and minimum bactericidal concentration (MBC) were 0.22, 0.11 and 0.11 mg/mL, respectively. The MICs remained steady under different pH values (3.0–9.0) and temperatures (4 and 25 °C), indicating that the anti-bacterial effect of MβCD-DG was independent of pH and temperature. Measurements of permeability of cell wall and membrane, membrane potential, release of cellular contents and observation of cell morphology were carried out to explore the antibacterial mechanism of MβCD-DG. The results indicated that MβCD-DG inhibited S. aureus by increasing the permeability of cell membrane and leakage of cellular contents without obvious cell morphological changes. MβCD-DG may kill B. cereus and B. subtilis by damaging the cell wall and cytoplasmic membrane to make the release of cellular contents. As a novel preservative, MβCD-DG is more efficient than DG and common chemical additives, and is thus worthy of further exploitation in food industry.",
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AU - Ma, Maomao

AU - Zeng, Zheling

AU - Yu, Ping

AU - Gong, Deming

AU - Deng, Shuguang

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N2 - The application of dodecyl gallate (DG) is limited due to its poor stability and water-solubility. A new compound of DG microencapsulated with methyl-β-cyclodextrin (MβCD), MβCD-DG was produced, and the inhibition effects of MβCD-DG against three common foodborne pathogens (Staphylococcus aureus, Bacillus cereus and Bacillus subtilis) were investigated in the study. The antioxidant activity of MβCD-DG was determined by the DPPH method (EC50 = 4.515 ± 0.5 μg/mL). The minimum inhibitory concentration (MIC) of MβCD-DG against these bacteria were 0.11, 0.06 and 0.06 mg/mL, and minimum bactericidal concentration (MBC) were 0.22, 0.11 and 0.11 mg/mL, respectively. The MICs remained steady under different pH values (3.0–9.0) and temperatures (4 and 25 °C), indicating that the anti-bacterial effect of MβCD-DG was independent of pH and temperature. Measurements of permeability of cell wall and membrane, membrane potential, release of cellular contents and observation of cell morphology were carried out to explore the antibacterial mechanism of MβCD-DG. The results indicated that MβCD-DG inhibited S. aureus by increasing the permeability of cell membrane and leakage of cellular contents without obvious cell morphological changes. MβCD-DG may kill B. cereus and B. subtilis by damaging the cell wall and cytoplasmic membrane to make the release of cellular contents. As a novel preservative, MβCD-DG is more efficient than DG and common chemical additives, and is thus worthy of further exploitation in food industry.

AB - The application of dodecyl gallate (DG) is limited due to its poor stability and water-solubility. A new compound of DG microencapsulated with methyl-β-cyclodextrin (MβCD), MβCD-DG was produced, and the inhibition effects of MβCD-DG against three common foodborne pathogens (Staphylococcus aureus, Bacillus cereus and Bacillus subtilis) were investigated in the study. The antioxidant activity of MβCD-DG was determined by the DPPH method (EC50 = 4.515 ± 0.5 μg/mL). The minimum inhibitory concentration (MIC) of MβCD-DG against these bacteria were 0.11, 0.06 and 0.06 mg/mL, and minimum bactericidal concentration (MBC) were 0.22, 0.11 and 0.11 mg/mL, respectively. The MICs remained steady under different pH values (3.0–9.0) and temperatures (4 and 25 °C), indicating that the anti-bacterial effect of MβCD-DG was independent of pH and temperature. Measurements of permeability of cell wall and membrane, membrane potential, release of cellular contents and observation of cell morphology were carried out to explore the antibacterial mechanism of MβCD-DG. The results indicated that MβCD-DG inhibited S. aureus by increasing the permeability of cell membrane and leakage of cellular contents without obvious cell morphological changes. MβCD-DG may kill B. cereus and B. subtilis by damaging the cell wall and cytoplasmic membrane to make the release of cellular contents. As a novel preservative, MβCD-DG is more efficient than DG and common chemical additives, and is thus worthy of further exploitation in food industry.

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