Anisotropic poly (glycerol sebacate)-poly (-caprolactone) electrospun fibers promote endothelial cell guidance

Akhilesh K. Gaharwar, Mehdi Nikkhah, Shilpa Sant, Ali Khademhosseini

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Topographical cell guidance is utilized to engineer highly organized and aligned cellular constructs for numerous tissue engineering applications. Recently, electrospun scaffolds fabricated using poly(glycerol sebacate) (PGS) and poly(-caprolactone) (PCL) have shown a great promise to support valvular interstitial cell functions for the development of tissue engineered heart valves. However, one of the major drawbacks of PGS-PCL scaffolds is the lack of control over cellular alignment. In this work, we investigate the role of scaffold architecture on the endothelial cell alignment, proliferation and formation of organized cellular structures. In particular, PGS-PCL scaffolds with randomly oriented and highly aligned fibers with tunable mechanical properties were fabricated using electrospinning technique. After one week of culture, endothelial cells on the aligned scaffolds exhibited higher proliferation compared to those cultures on randomly oriented fibrous scaffolds. Furthermore, the endothelial cells reorganized in response to the topographical features of aligned scaffolds forming highly organized cellular constructs. Thus, topographical contact guidance, provided by aligned PGS-PCL scaffolds, is envisioned to be useful in developing cellular structures for vascular tissue engineering.

Original languageEnglish (US)
Article number015001
JournalBiofabrication
Volume7
Issue number1
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Keywords

  • electrospun scaffolds
  • endothelial cells
  • poly (glycerol sebacate)
  • tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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