Abstract

This critical review presents challenges and strategies in the detection of viral contaminants in food products. Adenovirus, caliciviruses, enteroviruses, and hepatitis A are emerging contaminant viruses. These viruses contaminate a variety of food products, including fruits, vegetables, shellfish, and ready-to-eat processed foods. The diversity of targets and sample matrices presents unique challenges to virus monitoring that have been addressed by a wide array of processing and detection methods. This review covers sample acquisition and handling, virus recovery/concentration, and the determination of targets using molecular biology and mass-spectrometric approaches. The concentration methods discussed include precipitation, antibody-based concentration, and filtration; the detection methods discussed include microscopy, polymerase chain reaction, nucleic acid sequence-based amplification, and mass spectrometry.

Original languageEnglish (US)
Pages (from-to)2527-2537
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume404
Issue number9
DOIs
StatePublished - Nov 2012

Fingerprint

Viruses
Food
Self-Sustained Sequence Replication
Nucleic acid sequences
Processed foods
Impurities
Shellfish
Molecular biology
Hepatitis A
Enterovirus
Polymerase chain reaction
Vegetables
Molecular mass
Fruits
Adenoviridae
Mass spectrometry
Amplification
Molecular Biology
Microscopy
Fruit

Keywords

  • Adenovirus
  • Detection
  • Enterovirus
  • Hepatitis A
  • Norovirus
  • Processing

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Analytical methods for the detection of viruses in food by example of CCL-3 bioagents. / Hartmann, E. M.; Halden, Rolf.

In: Analytical and Bioanalytical Chemistry, Vol. 404, No. 9, 11.2012, p. 2527-2537.

Research output: Contribution to journalArticle

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