Analysis of the human serum proteome

King C. Chan, David A. Lucas, Denise Hise, Carl F. Schaefer, Zhen Xiao, George M. Janini, Kenneth Buetow, Haleem J. Issaq, Timothy D. Veenstra, Thomas P. Conrads

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Changes in serum proteins that signal histopathological states, such as cancer, are useful diagnostic and prognostic biomarkers. Unfortunately, the large dynamic concentration range of proteins in serum makes it a challenging proteome to effectively characterize. Typically, methods to deplete highly abundant proteins to decrease this dynamic protein concentration range are employed, yet such depletion results in removal of important low abundant proteins. A multi-dimensional peptide separation strategy utilizing conventional separation techniques combined with tandem mass spectrometry (MS/MS) was employed for a proteome analysis of human serum. Serum proteins were digested with trypsin and resolved into 20 fractions by ampholyte-free liquid phase isoelectric focusing. These 20 peptide fractions were further fractionated by strong cation-exchange chromatography, each of which was analyzed by microcapillary reversed-phase liquid chromatography coupled online with MS/MS analysis. This investigation resulted in the identification of 1444 unique proteins in serum. Proteins from all functional classes, cellular localization, and abundance levels were identified. This study illustrates that a majority of lower abundance proteins identified in serum are present as secreted or shed species by cells as a result of signalling, necrosis, apoptosis, and hemolysis. These findings show that the protein content of serum is quite reflective of the overall profile of the human organism and a conventional multi-dimensional fractionation strategy combined with MS/MS is entirely capable of characterizing a significant fraction of the serum proteome. We have constructed a publicly available human serum proteomic database (http://bpp.nci.nih.gov) to provide a reference resource to facilitate future investigations of the vast archive of pathophysiological content in serum.

Original languageEnglish (US)
Pages (from-to)101-225
Number of pages125
JournalClinical Proteomics
Volume1
Issue number2
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Proteome
Blood Proteins
Serum
Proteins
Peptides
Isoelectric Focusing
Reverse-Phase Chromatography
Tandem Mass Spectrometry
Hemolysis
Proteomics
Trypsin
Cations
Chromatography
Buffers
Necrosis
Liquid chromatography
Biomarkers
Fractionation
Databases
Apoptosis

Keywords

  • Biomarker
  • Isoelectric focusing
  • Mass spectrometry
  • Multi-dimensional separation
  • Proteomics
  • Serum

ASJC Scopus subject areas

  • Genetics
  • Molecular Medicine
  • Molecular Biology
  • Biochemistry, medical

Cite this

Chan, K. C., Lucas, D. A., Hise, D., Schaefer, C. F., Xiao, Z., Janini, G. M., ... Conrads, T. P. (2004). Analysis of the human serum proteome. Clinical Proteomics, 1(2), 101-225. https://doi.org/10.1385/CP:1:2:101

Analysis of the human serum proteome. / Chan, King C.; Lucas, David A.; Hise, Denise; Schaefer, Carl F.; Xiao, Zhen; Janini, George M.; Buetow, Kenneth; Issaq, Haleem J.; Veenstra, Timothy D.; Conrads, Thomas P.

In: Clinical Proteomics, Vol. 1, No. 2, 2004, p. 101-225.

Research output: Contribution to journalArticle

Chan, KC, Lucas, DA, Hise, D, Schaefer, CF, Xiao, Z, Janini, GM, Buetow, K, Issaq, HJ, Veenstra, TD & Conrads, TP 2004, 'Analysis of the human serum proteome', Clinical Proteomics, vol. 1, no. 2, pp. 101-225. https://doi.org/10.1385/CP:1:2:101
Chan KC, Lucas DA, Hise D, Schaefer CF, Xiao Z, Janini GM et al. Analysis of the human serum proteome. Clinical Proteomics. 2004;1(2):101-225. https://doi.org/10.1385/CP:1:2:101
Chan, King C. ; Lucas, David A. ; Hise, Denise ; Schaefer, Carl F. ; Xiao, Zhen ; Janini, George M. ; Buetow, Kenneth ; Issaq, Haleem J. ; Veenstra, Timothy D. ; Conrads, Thomas P. / Analysis of the human serum proteome. In: Clinical Proteomics. 2004 ; Vol. 1, No. 2. pp. 101-225.
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