TY - JOUR
T1 - Analysis of SOX2-expressing cell populations derived from human pluripotent stem cells
AU - Brafman, David A.
AU - Moya, Noel
AU - Allen-Soltero, Stephanie
AU - Fellner, Thomas
AU - Robinson, Megan
AU - McMillen, Zoë L.
AU - Gaasterland, Terry
AU - Willert, Karl
N1 - Funding Information:
We would like to thank Eric O’Connor of the UCSD/Sanford Consortium for Regenerative Medicine (SCRM) Human Embryonic Stem Cell Core for assistance with cell sorting and the SCRM Viral Vector Core for production of rAAV. D.A.B. was supported by funding from the UCSD Stem Cell Program and a gift from Michael and Nancy Kaehr. This research was supported in part by the National Institute of Diabetes and Digestive and Kidney Diseases Beta Cell Biology Consortium (5U01DK089567-02) and the California Institute for Regenerative Medicine (RT2-02064). This work was made possible in part by the CIRM Major Facilities grant (FA1-00607) to the Sanford Consortium for Regenerative Medicine.
PY - 2013/11/19
Y1 - 2013/11/19
N2 - SOX2 is involved in several cell and developmental processes, including maintenance of embryonic stem cells, differentiation of neural progenitor cells, and patterning of gut endoderm. To study its role in a human system, we generated a human embryonic stem cell (hESC) line harboring a reporter gene encoding GFP in the SOX2 locus. This SOX2 reporter line faithfully recapitulates expression of the SOX2 gene in undifferentiated human pluripotent stem cells (hPSCs), neural progenitor cells (NPCs), and anterior foregut endoderm (AFE). In undifferentiated hESCs, GFP expression corresponds to those cells with highest levels of expression of genes associated with the pluripotent state. In NPCs, expression of GFP can be employed to isolate cells expressing markers associated with NPC multipotency. In AFE, we used transcriptome-wide expression analysis to identify cell surface markers with elevated expression in this population, thereby facilitating isolation and purification of this hPSC-derived cell population.
AB - SOX2 is involved in several cell and developmental processes, including maintenance of embryonic stem cells, differentiation of neural progenitor cells, and patterning of gut endoderm. To study its role in a human system, we generated a human embryonic stem cell (hESC) line harboring a reporter gene encoding GFP in the SOX2 locus. This SOX2 reporter line faithfully recapitulates expression of the SOX2 gene in undifferentiated human pluripotent stem cells (hPSCs), neural progenitor cells (NPCs), and anterior foregut endoderm (AFE). In undifferentiated hESCs, GFP expression corresponds to those cells with highest levels of expression of genes associated with the pluripotent state. In NPCs, expression of GFP can be employed to isolate cells expressing markers associated with NPC multipotency. In AFE, we used transcriptome-wide expression analysis to identify cell surface markers with elevated expression in this population, thereby facilitating isolation and purification of this hPSC-derived cell population.
UR - http://www.scopus.com/inward/record.url?scp=84888131100&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84888131100&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2013.09.005
DO - 10.1016/j.stemcr.2013.09.005
M3 - Article
C2 - 24286033
AN - SCOPUS:84888131100
SN - 2213-6711
VL - 1
SP - 464
EP - 478
JO - Stem Cell Reports
JF - Stem Cell Reports
IS - 5
ER -