Analysis of SOX2-expressing cell populations derived from human pluripotent stem cells

David A. Brafman, Noel Moya, Stephanie Allen-Soltero, Thomas Fellner, Megan Robinson, Zoë L. McMillen, Terry Gaasterland, Karl Willert

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

SOX2 is involved in several cell and developmental processes, including maintenance of embryonic stem cells, differentiation of neural progenitor cells, and patterning of gut endoderm. To study its role in a human system, we generated a human embryonic stem cell (hESC) line harboring a reporter gene encoding GFP in the SOX2 locus. This SOX2 reporter line faithfully recapitulates expression of the SOX2 gene in undifferentiated human pluripotent stem cells (hPSCs), neural progenitor cells (NPCs), and anterior foregut endoderm (AFE). In undifferentiated hESCs, GFP expression corresponds to those cells with highest levels of expression of genes associated with the pluripotent state. In NPCs, expression of GFP can be employed to isolate cells expressing markers associated with NPC multipotency. In AFE, we used transcriptome-wide expression analysis to identify cell surface markers with elevated expression in this population, thereby facilitating isolation and purification of this hPSC-derived cell population.

Original languageEnglish (US)
Pages (from-to)464-478
Number of pages15
JournalStem Cell Reports
Volume1
Issue number5
DOIs
StatePublished - Nov 19 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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