Analysis of putative RNase P RNA from orthopoxviruses

Li Yang, Donna Wesolowski, Yong Li, Sidney Altman

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A putative RNase P RNA gene in camelpox virus, one of the orthopoxviruses, was cloned and transcribed in vitro. No RNase P activity could be detected in vitro from camelpox virus RNase P RNA alone, or by addition of the Escherichia coli RNase P protein subunit to reaction mixtures. Camelpox virus RNase P RNA reconstituted in vitro with camel or HeLa cell extracts, which were pre-treated with micrococcal nuclease to degrade the endogenous RNase P RNA, showed no RNase P activity. Vaccinia virus, another orthopoxvirus, showed no RNase P activity in vaccinia-infected HeLa cells, even though transcription of the vaccinia RNase P RNA could be identified in the cells by both Northern blot and RNase protection assay. Camelpox virus RNase P RNA inhibited an endogenous HeLa RNase P activity by 20% in our assays. The 5 S RNA showed no significant inhibition in this assay.

Original languageEnglish (US)
Pages (from-to)529-535
Number of pages7
JournalJournal of molecular biology
Volume354
Issue number3
DOIs
StatePublished - Dec 2 2005
Externally publishedYes

Keywords

  • Orthopoxviruses
  • RNase P RNA subunit
  • RNase P cleavage activity
  • tRNA

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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