Analysis of putative RNase P RNA from orthopoxviruses

Li Yang, Donna Wesolowski, Yong Li, Sidney Altman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A putative RNase P RNA gene in camelpox virus, one of the orthopoxviruses, was cloned and transcribed in vitro. No RNase P activity could be detected in vitro from camelpox virus RNase P RNA alone, or by addition of the Escherichia coli RNase P protein subunit to reaction mixtures. Camelpox virus RNase P RNA reconstituted in vitro with camel or HeLa cell extracts, which were pre-treated with micrococcal nuclease to degrade the endogenous RNase P RNA, showed no RNase P activity. Vaccinia virus, another orthopoxvirus, showed no RNase P activity in vaccinia-infected HeLa cells, even though transcription of the vaccinia RNase P RNA could be identified in the cells by both Northern blot and RNase protection assay. Camelpox virus RNase P RNA inhibited an endogenous HeLa RNase P activity by 20% in our assays. The 5 S RNA showed no significant inhibition in this assay.

Original languageEnglish (US)
Pages (from-to)529-535
Number of pages7
JournalJournal of Molecular Biology
Volume354
Issue number3
DOIs
StatePublished - Dec 2 2005
Externally publishedYes

Fingerprint

Orthopoxvirus
Ribonuclease P
RNA
Vaccinia
HeLa Cells
Micrococcal Nuclease
Camelus
Vaccinia virus
Protein Subunits
Ribonucleases
Cell Extracts
Northern Blotting

Keywords

  • Orthopoxviruses
  • RNase P cleavage activity
  • RNase P RNA subunit
  • tRNA

ASJC Scopus subject areas

  • Virology

Cite this

Analysis of putative RNase P RNA from orthopoxviruses. / Yang, Li; Wesolowski, Donna; Li, Yong; Altman, Sidney.

In: Journal of Molecular Biology, Vol. 354, No. 3, 02.12.2005, p. 529-535.

Research output: Contribution to journalArticle

Yang, Li ; Wesolowski, Donna ; Li, Yong ; Altman, Sidney. / Analysis of putative RNase P RNA from orthopoxviruses. In: Journal of Molecular Biology. 2005 ; Vol. 354, No. 3. pp. 529-535.
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