Analysis of Products Formed during Bleomycin-Mediated DNA Degradation†

Natesan Murugesan, Cheng Xu, Guy M. Ehrenfeld, Hiroshi Sugiyama, Robert E. Kilkuskie, Luis O. Rodriguez, Li Ho Chang, Sidney M. Hecht

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

By the use of DNA, copolymers of defined nucleotide composition, and a synthetic dodecanucleotide having putative bleomycin cleavage sites in proximity to the 5'- and 3'-termini, the products formed concomitant with DNA strand scission have been isolated and subjected to structural identification and quantitation via direct comparison with authentic synthetic samples. The products of DNA strand scission by Fe(II)-bleomycin include oligonucleotides having each of the four possible nucleoside 3'-(phosphoro-2"-O-glycolates) at their 3'-termini, as well as the four possible base propenals. At least for 3-(adenin-9'-yl)propenal and 3-(thymin-l'-yl)propenal, the products formed were exclusively of the trans configuration.

Original languageEnglish (US)
Pages (from-to)5735-5744
Number of pages10
JournalBiochemistry
Volume24
Issue number21
DOIs
StatePublished - Oct 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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