Analysis of Products Formed during Bleomycin-Mediated DNA Degradation†

Natesan Murugesan; Cheng Xu; Guy M. Ehrenfeld; Hiroshi Sugiyama; Robert E. Kilkuskie; Luis O. Rodriguez; Li Ho Chang; Sidney M. Hecht

doi:10.1021/bi00342a008

Analysis of Products Formed during Bleomycin-Mediated DNA Degradation†

Natesan Murugesan, Cheng Xu, Guy M. Ehrenfeld, Hiroshi Sugiyama, Robert E. Kilkuskie, Luis O. Rodriguez, Li Ho Chang, Sidney M. Hecht

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

By the use of DNA, copolymers of defined nucleotide composition, and a synthetic dodecanucleotide having putative bleomycin cleavage sites in proximity to the 5'- and 3'-termini, the products formed concomitant with DNA strand scission have been isolated and subjected to structural identification and quantitation via direct comparison with authentic synthetic samples. The products of DNA strand scission by Fe(II)-bleomycin include oligonucleotides having each of the four possible nucleoside 3'-(phosphoro-2"-O-glycolates) at their 3'-termini, as well as the four possible base propenals. At least for 3-(adenin-9'-yl)propenal and 3-(thymin-l'-yl)propenal, the products formed were exclusively of the trans configuration.

Original language	English (US)
Pages (from-to)	5735-5744
Number of pages	10
Journal	Biochemistry
Volume	24
Issue number	21
DOIs	https://doi.org/10.1021/bi00342a008
State	Published - Oct 1 1985
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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10.1021/bi00342a008

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title = "Analysis of Products Formed during Bleomycin-Mediated DNA Degradation†",

abstract = "By the use of DNA, copolymers of defined nucleotide composition, and a synthetic dodecanucleotide having putative bleomycin cleavage sites in proximity to the 5'- and 3'-termini, the products formed concomitant with DNA strand scission have been isolated and subjected to structural identification and quantitation via direct comparison with authentic synthetic samples. The products of DNA strand scission by Fe(II)-bleomycin include oligonucleotides having each of the four possible nucleoside 3'-(phosphoro-2{"}-O-glycolates) at their 3'-termini, as well as the four possible base propenals. At least for 3-(adenin-9'-yl)propenal and 3-(thymin-l'-yl)propenal, the products formed were exclusively of the trans configuration.",

author = "Natesan Murugesan and Cheng Xu and Ehrenfeld, {Guy M.} and Hiroshi Sugiyama and Kilkuskie, {Robert E.} and Rodriguez, {Luis O.} and Chang, {Li Ho} and Hecht, {Sidney M.}",

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T1 - Analysis of Products Formed during Bleomycin-Mediated DNA Degradation†

AU - Murugesan, Natesan

AU - Xu, Cheng

AU - Ehrenfeld, Guy M.

AU - Sugiyama, Hiroshi

AU - Kilkuskie, Robert E.

AU - Rodriguez, Luis O.

AU - Chang, Li Ho

AU - Hecht, Sidney M.

PY - 1985/10/1

Y1 - 1985/10/1

N2 - By the use of DNA, copolymers of defined nucleotide composition, and a synthetic dodecanucleotide having putative bleomycin cleavage sites in proximity to the 5'- and 3'-termini, the products formed concomitant with DNA strand scission have been isolated and subjected to structural identification and quantitation via direct comparison with authentic synthetic samples. The products of DNA strand scission by Fe(II)-bleomycin include oligonucleotides having each of the four possible nucleoside 3'-(phosphoro-2"-O-glycolates) at their 3'-termini, as well as the four possible base propenals. At least for 3-(adenin-9'-yl)propenal and 3-(thymin-l'-yl)propenal, the products formed were exclusively of the trans configuration.

AB - By the use of DNA, copolymers of defined nucleotide composition, and a synthetic dodecanucleotide having putative bleomycin cleavage sites in proximity to the 5'- and 3'-termini, the products formed concomitant with DNA strand scission have been isolated and subjected to structural identification and quantitation via direct comparison with authentic synthetic samples. The products of DNA strand scission by Fe(II)-bleomycin include oligonucleotides having each of the four possible nucleoside 3'-(phosphoro-2"-O-glycolates) at their 3'-termini, as well as the four possible base propenals. At least for 3-(adenin-9'-yl)propenal and 3-(thymin-l'-yl)propenal, the products formed were exclusively of the trans configuration.

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Analysis of Products Formed during Bleomycin-Mediated DNA Degradation†

Abstract

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