TY - JOUR
T1 - Analysis of Iranian Potato virus S isolates
AU - Salari, Khadijeh
AU - Massumi, Hossein
AU - Heydarnejad, Jahangir
AU - Hosseini Pour, Akbar
AU - Varsani, Arvind
N1 - Funding Information:
Acknowledgment This work was supported by a grant from Shahid Bahonar University of Kerman, Kerman, Iran.
PY - 2011/10
Y1 - 2011/10
N2 - Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a Potato virus S (PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on Nicotiana debneyii and N. tabacum cv. Whiteburly and local lesion on Chenopodium amaranticolor, C. quinoa and C. album. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS O isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS O strain and 3 genotypes for the PVS A strain.
AB - Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a Potato virus S (PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on Nicotiana debneyii and N. tabacum cv. Whiteburly and local lesion on Chenopodium amaranticolor, C. quinoa and C. album. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS O isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS O strain and 3 genotypes for the PVS A strain.
KW - Iran
KW - PVS genotypes
KW - Phylogenetic analysis
KW - Potato virus S
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U2 - 10.1007/s11262-011-0619-3
DO - 10.1007/s11262-011-0619-3
M3 - Article
C2 - 21567245
AN - SCOPUS:80052332472
SN - 0920-8569
VL - 43
SP - 281
EP - 288
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -