Analysis of Iranian Potato virus S isolates

Khadijeh Salari, Hossein Massumi, Jahangir Heydarnejad, Akbar Hosseini Pour, Arvind Varsani

Research output: Contribution to journalArticle

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Abstract

Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a Potato virus S (PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on Nicotiana debneyii and N. tabacum cv. Whiteburly and local lesion on Chenopodium amaranticolor, C. quinoa and C. album. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS O isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS O strain and 3 genotypes for the PVS A strain.

Original languageEnglish (US)
Pages (from-to)281-288
Number of pages8
JournalVirus Genes
Volume43
Issue number2
DOIs
StatePublished - Oct 2011
Externally publishedYes

Fingerprint

Solanum tuberosum
Viruses
Chenopodium
Chenopodium quinoa
Genotype
Antibodies
Capsid Proteins
Tobacco
Nucleotides
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Genes

Keywords

  • Iran
  • Phylogenetic analysis
  • Potato virus S
  • PVS genotypes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Salari, K., Massumi, H., Heydarnejad, J., Hosseini Pour, A., & Varsani, A. (2011). Analysis of Iranian Potato virus S isolates. Virus Genes, 43(2), 281-288. https://doi.org/10.1007/s11262-011-0619-3

Analysis of Iranian Potato virus S isolates. / Salari, Khadijeh; Massumi, Hossein; Heydarnejad, Jahangir; Hosseini Pour, Akbar; Varsani, Arvind.

In: Virus Genes, Vol. 43, No. 2, 10.2011, p. 281-288.

Research output: Contribution to journalArticle

Salari, K, Massumi, H, Heydarnejad, J, Hosseini Pour, A & Varsani, A 2011, 'Analysis of Iranian Potato virus S isolates', Virus Genes, vol. 43, no. 2, pp. 281-288. https://doi.org/10.1007/s11262-011-0619-3
Salari K, Massumi H, Heydarnejad J, Hosseini Pour A, Varsani A. Analysis of Iranian Potato virus S isolates. Virus Genes. 2011 Oct;43(2):281-288. https://doi.org/10.1007/s11262-011-0619-3
Salari, Khadijeh ; Massumi, Hossein ; Heydarnejad, Jahangir ; Hosseini Pour, Akbar ; Varsani, Arvind. / Analysis of Iranian Potato virus S isolates. In: Virus Genes. 2011 ; Vol. 43, No. 2. pp. 281-288.
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