Analysis of host specificity of two closely related baculoviruses in permissive and nonpermissive cell lines

Md Masmudur Rahman, Karumathil P. Gopinathan

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The baculoviruses Bombyx mori nucleopolyhedrovirus (BmNPV) and Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) share about 90% identity at the genomic level but they have non-overlapping host range and show a high degree of host specificity. We have demonstrated here that AcMNPV undergoes DNA replication and early gene expression in Bombyx-derived BmN cells but fails to show very late gene expression or produce budded virion (BV) particles. Coinfection with BmNPV supported BV production from AcMNPV in BmN cells at low levels but not very late gene expression or polyhedral inclusion body formation. BV production and very late gene expression from BmNPV, on the contrary, were adversely affected in coinfections. In Spodoptera frugiperda-derived Sf21 cell lines, BmNPV DNA replication, BV production, and very late gene expression took place only when coinfected with AcMNPV. BmNPV exerted a less profound effect on AcMNPV multiplication and very late gene expression in permissive host cell lines. AcMNPV shuts down cellular and viral protein synthesis completely when infected alone or coinfected with BmNPV in BmN cells, whereas BmNPV infection did not affect cellular and viral protein synthesis in Sf21 cells. Overall, AcMNPV showed a more dominant effect by complementing the multiplication of BmNPV in nonpermissive host cells while inhibiting it in BmN cells.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalVirus research
Volume93
Issue number1
DOIs
StatePublished - May 1 2003
Externally publishedYes

Keywords

  • AcMNPV
  • Baculovirus
  • BmNPV
  • Host range
  • Virus infection process

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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