Analysis of host specificity of two closely related baculoviruses in permissive and nonpermissive cell lines

Masmudur Rahman, Karumathil P. Gopinathan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The baculoviruses Bombyx mori nucleopolyhedrovirus (BmNPV) and Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) share about 90% identity at the genomic level but they have non-overlapping host range and show a high degree of host specificity. We have demonstrated here that AcMNPV undergoes DNA replication and early gene expression in Bombyx-derived BmN cells but fails to show very late gene expression or produce budded virion (BV) particles. Coinfection with BmNPV supported BV production from AcMNPV in BmN cells at low levels but not very late gene expression or polyhedral inclusion body formation. BV production and very late gene expression from BmNPV, on the contrary, were adversely affected in coinfections. In Spodoptera frugiperda-derived Sf21 cell lines, BmNPV DNA replication, BV production, and very late gene expression took place only when coinfected with AcMNPV. BmNPV exerted a less profound effect on AcMNPV multiplication and very late gene expression in permissive host cell lines. AcMNPV shuts down cellular and viral protein synthesis completely when infected alone or coinfected with BmNPV in BmN cells, whereas BmNPV infection did not affect cellular and viral protein synthesis in Sf21 cells. Overall, AcMNPV showed a more dominant effect by complementing the multiplication of BmNPV in nonpermissive host cells while inhibiting it in BmN cells.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalVirus Research
Volume93
Issue number1
DOIs
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Nucleopolyhedrovirus
Baculoviridae
Host Specificity
Bombyx
Cell Line
Gene Expression
Virion
Sf9 Cells
Viral Proteins
DNA Replication
Coinfection
Spodoptera
Inclusion Bodies

Keywords

  • AcMNPV
  • Baculovirus
  • BmNPV
  • Host range
  • Virus infection process

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Analysis of host specificity of two closely related baculoviruses in permissive and nonpermissive cell lines. / Rahman, Masmudur; Gopinathan, Karumathil P.

In: Virus Research, Vol. 93, No. 1, 01.05.2003, p. 13-23.

Research output: Contribution to journalArticle

@article{7475b85012c349b1825cc08abbb4099a,
title = "Analysis of host specificity of two closely related baculoviruses in permissive and nonpermissive cell lines",
abstract = "The baculoviruses Bombyx mori nucleopolyhedrovirus (BmNPV) and Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) share about 90{\%} identity at the genomic level but they have non-overlapping host range and show a high degree of host specificity. We have demonstrated here that AcMNPV undergoes DNA replication and early gene expression in Bombyx-derived BmN cells but fails to show very late gene expression or produce budded virion (BV) particles. Coinfection with BmNPV supported BV production from AcMNPV in BmN cells at low levels but not very late gene expression or polyhedral inclusion body formation. BV production and very late gene expression from BmNPV, on the contrary, were adversely affected in coinfections. In Spodoptera frugiperda-derived Sf21 cell lines, BmNPV DNA replication, BV production, and very late gene expression took place only when coinfected with AcMNPV. BmNPV exerted a less profound effect on AcMNPV multiplication and very late gene expression in permissive host cell lines. AcMNPV shuts down cellular and viral protein synthesis completely when infected alone or coinfected with BmNPV in BmN cells, whereas BmNPV infection did not affect cellular and viral protein synthesis in Sf21 cells. Overall, AcMNPV showed a more dominant effect by complementing the multiplication of BmNPV in nonpermissive host cells while inhibiting it in BmN cells.",
keywords = "AcMNPV, Baculovirus, BmNPV, Host range, Virus infection process",
author = "Masmudur Rahman and Gopinathan, {Karumathil P.}",
year = "2003",
month = "5",
day = "1",
doi = "10.1016/S0168-1702(03)00046-7",
language = "English (US)",
volume = "93",
pages = "13--23",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Analysis of host specificity of two closely related baculoviruses in permissive and nonpermissive cell lines

AU - Rahman, Masmudur

AU - Gopinathan, Karumathil P.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - The baculoviruses Bombyx mori nucleopolyhedrovirus (BmNPV) and Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) share about 90% identity at the genomic level but they have non-overlapping host range and show a high degree of host specificity. We have demonstrated here that AcMNPV undergoes DNA replication and early gene expression in Bombyx-derived BmN cells but fails to show very late gene expression or produce budded virion (BV) particles. Coinfection with BmNPV supported BV production from AcMNPV in BmN cells at low levels but not very late gene expression or polyhedral inclusion body formation. BV production and very late gene expression from BmNPV, on the contrary, were adversely affected in coinfections. In Spodoptera frugiperda-derived Sf21 cell lines, BmNPV DNA replication, BV production, and very late gene expression took place only when coinfected with AcMNPV. BmNPV exerted a less profound effect on AcMNPV multiplication and very late gene expression in permissive host cell lines. AcMNPV shuts down cellular and viral protein synthesis completely when infected alone or coinfected with BmNPV in BmN cells, whereas BmNPV infection did not affect cellular and viral protein synthesis in Sf21 cells. Overall, AcMNPV showed a more dominant effect by complementing the multiplication of BmNPV in nonpermissive host cells while inhibiting it in BmN cells.

AB - The baculoviruses Bombyx mori nucleopolyhedrovirus (BmNPV) and Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) share about 90% identity at the genomic level but they have non-overlapping host range and show a high degree of host specificity. We have demonstrated here that AcMNPV undergoes DNA replication and early gene expression in Bombyx-derived BmN cells but fails to show very late gene expression or produce budded virion (BV) particles. Coinfection with BmNPV supported BV production from AcMNPV in BmN cells at low levels but not very late gene expression or polyhedral inclusion body formation. BV production and very late gene expression from BmNPV, on the contrary, were adversely affected in coinfections. In Spodoptera frugiperda-derived Sf21 cell lines, BmNPV DNA replication, BV production, and very late gene expression took place only when coinfected with AcMNPV. BmNPV exerted a less profound effect on AcMNPV multiplication and very late gene expression in permissive host cell lines. AcMNPV shuts down cellular and viral protein synthesis completely when infected alone or coinfected with BmNPV in BmN cells, whereas BmNPV infection did not affect cellular and viral protein synthesis in Sf21 cells. Overall, AcMNPV showed a more dominant effect by complementing the multiplication of BmNPV in nonpermissive host cells while inhibiting it in BmN cells.

KW - AcMNPV

KW - Baculovirus

KW - BmNPV

KW - Host range

KW - Virus infection process

UR - http://www.scopus.com/inward/record.url?scp=0037408471&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037408471&partnerID=8YFLogxK

U2 - 10.1016/S0168-1702(03)00046-7

DO - 10.1016/S0168-1702(03)00046-7

M3 - Article

C2 - 12727338

AN - SCOPUS:0037408471

VL - 93

SP - 13

EP - 23

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 1

ER -