TY - JOUR
T1 - Analysis of autoantibodies to T-cell receptors among HIV-infected individuals
T2 - Epitope analysis and time course
AU - Marchalonis, John J.
AU - Ampel, Neil M.
AU - Schluter, Samuel F.
AU - Garza, Andrea
AU - Lake, Douglas F.
AU - Galgiani, John N.
AU - Landsperger, William J.
N1 - Funding Information:
This research was supported in part by grants from Arizona Disease Control Research Commission, Baxter Biotechnology/Hyland Division to J.J.M., and research supported in part by the U.S. Of®ce of Veterans Affairs to N.A. and J.G.
PY - 1997/2
Y1 - 1997/2
N2 - Individuals seropositive for human immunodeficiency virus type 1 (HIV) express elevated levels of autoantibodies (AAbs) directed against recombinant T-cell receptors (TCRs) and synthetic peptide epitopes duplicating β chain markers. We performed longitudinal studies of anti-TCR AAbs in HIV-1-infected individuals, making comparisons with uninfected sera and sera from other individuals infected with a nonviral agent. We determined levels of autoantibodies by titration using enzyme-linked immunosorbent assay (ELISA) and developed a means for characterizing 'autoantibody CDR recognition spectrotypes' for individual sera. Antibody levels against certain defined synthetic epitopes were substantially elevated in HIV-infected subjects relative to reactivities by control groups. Individual sera showed relatively high AAb levels to a subset of CDR1 peptide epitopes. Two patients who subsequently developed AIDS showed particular reactivity to Vβ2.1, 8.1, 10.1, and 22.1 epitopes. Our results show that production AAbs to TCR Vβ epitopes is a general consequence of HIV infection. The response is individual but shows some restriction and shifts in AAb subpopulations often occur with time.
AB - Individuals seropositive for human immunodeficiency virus type 1 (HIV) express elevated levels of autoantibodies (AAbs) directed against recombinant T-cell receptors (TCRs) and synthetic peptide epitopes duplicating β chain markers. We performed longitudinal studies of anti-TCR AAbs in HIV-1-infected individuals, making comparisons with uninfected sera and sera from other individuals infected with a nonviral agent. We determined levels of autoantibodies by titration using enzyme-linked immunosorbent assay (ELISA) and developed a means for characterizing 'autoantibody CDR recognition spectrotypes' for individual sera. Antibody levels against certain defined synthetic epitopes were substantially elevated in HIV-infected subjects relative to reactivities by control groups. Individual sera showed relatively high AAb levels to a subset of CDR1 peptide epitopes. Two patients who subsequently developed AIDS showed particular reactivity to Vβ2.1, 8.1, 10.1, and 22.1 epitopes. Our results show that production AAbs to TCR Vβ epitopes is a general consequence of HIV infection. The response is individual but shows some restriction and shifts in AAb subpopulations often occur with time.
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U2 - 10.1006/clin.1996.4290
DO - 10.1006/clin.1996.4290
M3 - Article
C2 - 9000486
AN - SCOPUS:0031081060
SN - 0090-1229
VL - 82
SP - 174
EP - 189
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -