Analysis of a challenging subset of world anti-doping agency-banned steroids and antiestrogens by LC-MS-MS

Chad R. Borges; Nicole Miller; Melinda Shelby; Megan Hansen; Claudia White; Matthew H. Slawson; Kim Monti; Dennis J. Crouch

doi:10.1093/jat/31.3.125

Analysis of a challenging subset of world anti-doping agency-banned steroids and antiestrogens by LC-MS-MS

Chad R. Borges, Nicole Miller, Melinda Shelby, Megan Hansen, Claudia White, Matthew H. Slawson, Kim Monti, Dennis J. Crouch

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A qualitative liquid chromatography-tandem mass spectrometry method for the analysis of 22 sporting federation-banned anabolic agents (or their metabolite markers) and anti-estrogens in urine that are refractory to analysis by gas chromatography-mass spectrometry is presented. In addition, a quantitative method built around World Anti-Doping Agency (WADA) guidelines for the confirmatory analysis of 19-norandrosterone, the primary metabolite of nandrolone with a WADA-specified minimum required performance limit of 1 ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid-liquid extraction, no clean-up derivatization with Girard's Reagent P, and analysis by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity well beyond that required by the WADA.

Original language	English (US)
Pages (from-to)	125-131
Number of pages	7
Journal	Journal of Analytical Toxicology
Volume	31
Issue number	3
DOIs	https://doi.org/10.1093/jat/31.3.125
State	Published - Apr 2007
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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abstract = "A qualitative liquid chromatography-tandem mass spectrometry method for the analysis of 22 sporting federation-banned anabolic agents (or their metabolite markers) and anti-estrogens in urine that are refractory to analysis by gas chromatography-mass spectrometry is presented. In addition, a quantitative method built around World Anti-Doping Agency (WADA) guidelines for the confirmatory analysis of 19-norandrosterone, the primary metabolite of nandrolone with a WADA-specified minimum required performance limit of 1 ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid-liquid extraction, no clean-up derivatization with Girard's Reagent P, and analysis by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity well beyond that required by the WADA.",

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AU - Borges, Chad R.

AU - Miller, Nicole

AU - Shelby, Melinda

AU - Hansen, Megan

AU - White, Claudia

AU - Slawson, Matthew H.

AU - Monti, Kim

AU - Crouch, Dennis J.

PY - 2007/4

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Analysis of a challenging subset of world anti-doping agency-banned steroids and antiestrogens by LC-MS-MS

Abstract

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