Analysis of a challenging subset of world anti-doping agency-banned steroids and antiestrogens by LC-MS-MS

Chad R. Borges, Nicole Miller, Melinda Shelby, Megan Hansen, Claudia White, Matthew H. Slawson, Kim Monti, Dennis J. Crouch

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A qualitative liquid chromatography-tandem mass spectrometry method for the analysis of 22 sporting federation-banned anabolic agents (or their metabolite markers) and anti-estrogens in urine that are refractory to analysis by gas chromatography-mass spectrometry is presented. In addition, a quantitative method built around World Anti-Doping Agency (WADA) guidelines for the confirmatory analysis of 19-norandrosterone, the primary metabolite of nandrolone with a WADA-specified minimum required performance limit of 1 ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid-liquid extraction, no clean-up derivatization with Girard's Reagent P, and analysis by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity well beyond that required by the WADA.

Original languageEnglish (US)
Pages (from-to)125-131
Number of pages7
JournalJournal of Analytical Toxicology
Volume31
Issue number3
DOIs
StatePublished - Apr 2007
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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