Abstract

Anaerobic oxidation of methane (AOM) is an important process for understanding the global flux of methane and its relation to the global carbon cycle. Although AOM is known to be coupled to reductions of sulfate, nitrite, and nitrate, evidence that AOM is coupled with extracellular electron transfer (EET) to conductive solids is relatively insufficient. Here, we demonstrate EET-dependent AOM in a biofilm anode dominated by Geobacter spp. and Methanobacterium spp. using carbon-fiber electrodes as the terminal electron sink. The steady-state current density was kept at 11.0 ± 1.3 mA/m2 in a microbial electrochemical cell, and isotopic experiments supported AOM-EET to the anode. Fluorescence in situ hybridization images and metagenome results suggest that Methanobacterium spp. may work synergistically with Geobacter spp. to allow AOM, likely by employing intermediate (formate or H2)-dependent inter-species electron transport. Since metal oxides are widely present in sedimentary and terrestrial environments, an AOM-EET niche would have implications for minimizing the net global emissions of methane.

Original languageEnglish (US)
Article number5099
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Methane
Electrodes
Electrons
Geobacter
Methanobacterium
formic acid
Metagenome
Carbon Cycle
Biofilms
Electron Transport
Nitrites
Fluorescence In Situ Hybridization
Nitrates
Oxides
Sulfates
Metals

ASJC Scopus subject areas

  • General

Cite this

Anaerobic oxidation of methane coupled with extracellular electron transfer to electrodes /631/61/168 /631/326/2522 /14/32 article. / Gao, Yaohuan; Lee, Jangho; Neufeld, Josh D.; Park, Joonhong; Rittmann, Bruce; Lee, Hyung Sool.

In: Scientific Reports, Vol. 7, No. 1, 5099, 01.12.2017.

Research output: Contribution to journalArticle

Gao, Yaohuan ; Lee, Jangho ; Neufeld, Josh D. ; Park, Joonhong ; Rittmann, Bruce ; Lee, Hyung Sool. / Anaerobic oxidation of methane coupled with extracellular electron transfer to electrodes /631/61/168 /631/326/2522 /14/32 article. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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