An unusual organization of the genes encoding cytochrome b559 in Chlamydomonas reinhardtii

psbE and psbF genes are separately transcribed from different regions of the plastid chromosome

Tsafrir Leket-Mor, Itzhak Ohad, Joseph Hirschberg, Himadri B. Pakrasi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The psbE and psbF genes encode the apoproteins of cytochrome b559, an essential component of the pigment protein complex photosystem II. Together with psbL and psbJ, these genes constitute a single operon in all photosynthetic organisms examined thus far. We have cloned and sequenced the psbE and psbF genes of the Chlamydomonas reinhardtii plastid genome. The predicted amino-terminal domains of both polypeptides are more basic than those of other organisms, and the sequence of the psbE gene product indicates a departure from the 'positive-inside' rule for the insertion of proteins in the thylakoid membrane. Northern blot analysis demonstrated that psbE is transcribed into a 0.3 kb mRNA, while transcription of psbF and psbL genes results in a 0.9 kb transcript. The splitting of the psbEFLJ operon into separate transcription units suggests a unique mechanism of regulation of expression of these genes in C. reinhardtii.

Original languageEnglish (US)
Pages (from-to)600-604
Number of pages5
JournalMGG Molecular & General Genetics
Volume246
Issue number5
DOIs
StatePublished - Sep 1995
Externally publishedYes

Fingerprint

Chlamydomonas reinhardtii
Plastids
Chromosomes
Genes
Operon
Thylakoid Membrane Proteins
Plastid Genomes
Apoproteins
Photosystem II Protein Complex
Gene Expression Regulation
Northern Blotting
cytochrome b559
Messenger RNA
Peptides

Keywords

  • Chlamydomonas reinhardtii
  • Cytochrome b
  • Photosystem II Chloroplast gene expression

ASJC Scopus subject areas

  • Genetics

Cite this

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title = "An unusual organization of the genes encoding cytochrome b559 in Chlamydomonas reinhardtii: psbE and psbF genes are separately transcribed from different regions of the plastid chromosome",
abstract = "The psbE and psbF genes encode the apoproteins of cytochrome b559, an essential component of the pigment protein complex photosystem II. Together with psbL and psbJ, these genes constitute a single operon in all photosynthetic organisms examined thus far. We have cloned and sequenced the psbE and psbF genes of the Chlamydomonas reinhardtii plastid genome. The predicted amino-terminal domains of both polypeptides are more basic than those of other organisms, and the sequence of the psbE gene product indicates a departure from the 'positive-inside' rule for the insertion of proteins in the thylakoid membrane. Northern blot analysis demonstrated that psbE is transcribed into a 0.3 kb mRNA, while transcription of psbF and psbL genes results in a 0.9 kb transcript. The splitting of the psbEFLJ operon into separate transcription units suggests a unique mechanism of regulation of expression of these genes in C. reinhardtii.",
keywords = "Chlamydomonas reinhardtii, Cytochrome b, Photosystem II Chloroplast gene expression",
author = "Tsafrir Leket-Mor and Itzhak Ohad and Joseph Hirschberg and Pakrasi, {Himadri B.}",
year = "1995",
month = "9",
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language = "English (US)",
volume = "246",
pages = "600--604",
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TY - JOUR

T1 - An unusual organization of the genes encoding cytochrome b559 in Chlamydomonas reinhardtii

T2 - psbE and psbF genes are separately transcribed from different regions of the plastid chromosome

AU - Leket-Mor, Tsafrir

AU - Ohad, Itzhak

AU - Hirschberg, Joseph

AU - Pakrasi, Himadri B.

PY - 1995/9

Y1 - 1995/9

N2 - The psbE and psbF genes encode the apoproteins of cytochrome b559, an essential component of the pigment protein complex photosystem II. Together with psbL and psbJ, these genes constitute a single operon in all photosynthetic organisms examined thus far. We have cloned and sequenced the psbE and psbF genes of the Chlamydomonas reinhardtii plastid genome. The predicted amino-terminal domains of both polypeptides are more basic than those of other organisms, and the sequence of the psbE gene product indicates a departure from the 'positive-inside' rule for the insertion of proteins in the thylakoid membrane. Northern blot analysis demonstrated that psbE is transcribed into a 0.3 kb mRNA, while transcription of psbF and psbL genes results in a 0.9 kb transcript. The splitting of the psbEFLJ operon into separate transcription units suggests a unique mechanism of regulation of expression of these genes in C. reinhardtii.

AB - The psbE and psbF genes encode the apoproteins of cytochrome b559, an essential component of the pigment protein complex photosystem II. Together with psbL and psbJ, these genes constitute a single operon in all photosynthetic organisms examined thus far. We have cloned and sequenced the psbE and psbF genes of the Chlamydomonas reinhardtii plastid genome. The predicted amino-terminal domains of both polypeptides are more basic than those of other organisms, and the sequence of the psbE gene product indicates a departure from the 'positive-inside' rule for the insertion of proteins in the thylakoid membrane. Northern blot analysis demonstrated that psbE is transcribed into a 0.3 kb mRNA, while transcription of psbF and psbL genes results in a 0.9 kb transcript. The splitting of the psbEFLJ operon into separate transcription units suggests a unique mechanism of regulation of expression of these genes in C. reinhardtii.

KW - Chlamydomonas reinhardtii

KW - Cytochrome b

KW - Photosystem II Chloroplast gene expression

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U2 - 10.1007/BF00298966

DO - 10.1007/BF00298966

M3 - Article

VL - 246

SP - 600

EP - 604

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 5

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